ArticleThe EMBO journal2007
The EM structure of human DNA polymerase gamma reveals a localized contact between the catalytic and accessory subunits.
Article in The EMBO journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- DNA polymerase β outperforms DNA polymerase γ in key mitochondrial base excision repair activities.DNA repair · 2021Article
- Quaternary structural diversity in eukaryotic DNA polymerases: monomeric to multimeric form.Current genetics · 2020Review
- Structure and function relationships in mammalian DNA polymerases.Cellular and molecular life sciences : CMLS · 2020Review
- Mitochondrial DNA maintenance: an appraisal.Molecular and cellular biochemistry · 2015Review
- Biochemical analysis of human POLG2 variants associated with mitochondrial disease.Human molecular genetics · 2011Article
- Mitochondrial disorders of DNA polymerase γ dysfunction: from anatomic to molecular pathology diagnosis.Archives of pathology & laboratory medicine · 2011Review
- POLG1 p.R722H mutation associated with multiple mtDNA deletions and a neurological phenotype.BMC neurology · 2010Article
- A mechanistic view of human mitochondrial DNA polymerase gamma: providing insight into drug toxicity and mitochondrial disease.Biochimica et biophysica acta · 2010Review
- POLG DNA testing as an emerging standard of care before instituting valproic acid therapy for pediatric seizure disorders.Seizure · 2010Article
- Article
- Twinkle mutations associated with autosomal dominant progressive external ophthalmoplegia lead to impaired helicase function and in vivo mtDNA replication stalling.Human molecular genetics · 2009Article
Corrections and comments
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Authors and funding
8 authors at 6 institutions in 2 countries.
Funding
Abstract
We used electron microscopy to examine the structure of human DNA pol gamma, the heterotrimeric mtDNA replicase implicated in certain mitochondrial diseases and aging models. Separate analysis of negatively stained preparations of the catalytic subunit, pol gammaA, and of the holoenzyme including a dimeric accessory factor, pol gammaB(2), permitted unambiguous identification of the position of the accessory factor within the holoenzyme. The model explains protection of a partial chymotryptic cleavage site after residue L(549) of pol gammaA upon binding of the accessory subunit. This interaction region is near residue 467 of pol gammaA, where a disease-related mutation has been reported to impair binding of the B subunit. One pol gammaB subunit dominates contacts with the catalytic subunit, while the second B subunit is largely exposed to solvent. A model for pol gamma is discussed that considers the effects of known mutations in the accessory subunit and the interaction of the enzyme with DNA.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.