ArticleThe Journal of membrane biology1991
Angiotensin II, vasopressin and GTP[gamma-S] inhibit inward-rectifying K+ channels in porcine cerebral capillary endothelial cells.
Article in The Journal of membrane biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 67 citations in OpenAlex.
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- Activation of VNeuropharmacology · 2021Article
- Capillary response to skeletal muscle contraction: evidence that redundancy between vasodilators is physiologically relevant during active hyperaemia.The Journal of physiology · 2018Article
- Boosting the signal: Endothelial inward rectifier KMicrocirculation (New York, N.Y. : 1994) · 2017Review
- Fluid and ion transfer across the blood-brain and blood-cerebrospinal fluid barriers; a comparative account of mechanisms and roles.Fluids and barriers of the CNS · 2016Review
- Blood-brain barrier KCa3.1 channels: evidence for a role in brain Na uptake and edema in ischemic stroke.Stroke · 2015Article
- Ion transporters in brain endothelial cells that contribute to formation of brain interstitial fluid.Pflugers Archiv : European journal of physiology · 2014Article
- Kv1 and Kir2 potassium channels are expressed in rat brain endothelial cells.Pflugers Archiv : European journal of physiology · 2008Article
- Involvement of Src tyrosine kinase and mitogen-activated protein kinase in the facilitation of calcium channels in rat nucleus of the tractus solitarius by angiotensin II.The Journal of physiology · 2005Article
- Ca(2+)- and voltage-dependent gating of Ca(2+)- and ATP-sensitive cationic channels in brain capillary endothelium.Biophysical journal · 2003Article
- An endogenous inactivating inward-rectifying potassium current in oocytes of Xenopus laevis.Pflugers Archiv : European journal of physiology · 1996Article
- Inwardly rectifying K+ channels in freshly dissociated coronary endothelial cells from guinea-pig heart.The Journal of physiology · 1996Article
- Physiological and molecular characterization of an IRK-type inward rectifier K+ channel in a tumour mast cell line.Pflugers Archiv : European journal of physiology · 1995Article
- Primary structure and characterization of a small-conductance inwardly rectifying potassium channel from human hippocampus.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- An electrophysiological study of angiotensin II regulation of Na-HCO3 cotransport and K conductance in renal proximal tubules. II. Effect of micromolar concentrations.Pflugers Archiv : European journal of physiology · 1994Article
- Ca2+ influx through stretch-activated cation channels activates maxi K+ channels in porcine endocardial endothelium.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Modulation by histamine of an inwardly rectifying potassium channel in human endothelial cells.The Journal of physiology · 1993Article
- Stretch-activated non-selective cation channels in the antiluminal membrane of porcine cerebral capillaries.The Journal of physiology · 1992Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cerebral capillaries from porcine brain were isolated, and endothelial cells were grown in primary culture. The whole-cell tight seal patch-clamp method was applied to freshly isolated single endothelial cells, and cells which were held in culture up to one week. With high K+ solution in the patch pipette and in the bath we observed inward-rectifying K+ currents, showing a time-dependent decay in part of the experiments. Ba2+ (1-10 mM) in the bath blocked this current, whereas outside tetraethylammonium (10 mM) decreased the peak current but increased the steady-state current. Addition of 1 microM of angiotensin II or of arginine-vasopressin to the extracellular side caused a time-dependent inhibition of the inward-rectifying K+ current in part of the experiments. Addition of 100 microM GTP[gamma-S] to the patch pipette blocked the K+ inward rectifier. In cell-attached membrane patches two types of single inward-rectifying K+ channels were observed, with single channel conductances of 7 and 35 pS. Cell-attached patches were also obtained at the antiluminal membrane of intact isolated cerebral capillaries. Only one type of K+ channel with g = 30 pS was recorded. In conclusion, inwardly rectifying K+ channels, which can be inhibited by extracellular angiotensin II and arginine-vasopressin, are present in cerebral capillary endothelial cells. The inhibition of this K+ conductance by GTP[gamma-S] indicates that G-proteins are involved in channel regulation. It is suggested that angiotensin II and vasopressin regulate K+ transport across the blood-brain barrier, mediating their effects via G-proteins.
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