ArticleBioelectrochemistry (Amsterdam, Netherlands)2007
Electric field-mediated transport of plasmid DNA in tumor interstitium in vivo.
Article in Bioelectrochemistry (Amsterdam, Netherlands), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- The impact of impaired DNA mobility on gene electrotransfer efficiency: analysis in 3D model.Biomedical engineering online · 2021Article
- Involvement of a Rac1-Dependent Macropinocytosis Pathway in Plasmid DNA Delivery by Electrotransfection.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
- New insights into the mechanisms of gene electrotransfer--experimental and theoretical analysis.Scientific reports · 2015Article
- In vivo molecular imaging and histological analysis of changes induced by electric pulses used for plasmid DNA electrotransfer to the skin: a study in a dorsal window chamber in mice.The Journal of membrane biology · 2012Article
- Enhancement of electric field-mediated gene delivery through pretreatment of tumors with a hyperosmotic mannitol solution.Cancer gene therapy · 2011Article
- Membrane binding of plasmid DNA and endocytic pathways are involved in electrotransfection of mammalian cells.PloS one · 2011Article
- The role of electrophoresis in gene electrotransfer.The Journal of membrane biology · 2010Article
- Article
- Relaxin treatment of solid tumors: effects on electric field-mediated gene delivery.Molecular cancer therapeutics · 2008Article
- Mechanistic analysis of electroporation-induced cellular uptake of macromolecules.Experimental biology and medicine (Maywood, N.J.) · 2008Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Local pulsed electric field application is a method for improving non-viral gene delivery. Mechanisms of the improvement include electroporation and electrophoresis. To understand how electrophoresis affects pDNA delivery in vivo, we quantified the magnitude of electric field-induced interstitial transport of pDNA in 4T1 and B16.F10 tumors implanted in mouse dorsal skin-fold chambers. Four different electric pulse sequences were used in this study, each consisted of 10 identical pulses that were 100 or 400 V/cm in strength and 20 or 50 ms in duration. The interval between consecutive pulses was 1 s. The largest distance of transport was obtained with the 400 V/cm and 50 ms pulse, and was 0.23 and 0.22 microm/pulse in 4T1 and B16.F10 tumors, respectively. There were no significant differences in transport distances between 4T1 and B16.F10 tumors. Results from in vivo mapping and numerical simulations revealed an approximately uniform intratumoral electric field that was predominantly in the direction of the applied field. The data in the study suggested that interstitial transport of pDNA induced by a sequence of ten electric pulses was ineffective for macroscopic delivery of genes in tumors. However, the induced transport was more efficient than passive diffusion.
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