ArticleParasitology research2007
Effects of serine protease inhibitors on viability and morphology of Leishmania (Leishmania) amazonensis promastigotes.
Article in Parasitology research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 61 citations in OpenAlex.
- Cnidarian Venoms in the Omics Era: Toxin Discovery and Bioactive Potential.Marine drugs · 2026Review
- Pretreatment with serine protease inhibitors impairs Leishmania amazonensis survival on macrophages.Parasites & vectors · 2025Article
- Polymeric nanoparticles containing kojic acid induce structural alterations and apoptosis-like death inFrontiers in pharmacology · 2024Article
- Hemoglobin uptake and utilization by human protozoan parasites: a review.Frontiers in cellular and infection microbiology · 2023Review
- Effects of a Serine Protease Inhibitor N-Pharmaceutics · 2022Article
- Activity of alkaloids from Aspidosperma nitidum against Leishmania (Leishmania) amazonensis.Scientific reports · 2022Article
- Subtilisin ofCurrent issues in molecular biology · 2022Article
- PF-429242, a Subtilisin Inhibitor, Is EffectiveFrontiers in microbiology · 2021Article
- Coccinia grandis (L.) Voigt Leaf Extract Exhibits Antileishmanial Effect Through Pro-inflammatory Response: An In Vitro Study.Current microbiology · 2017Article
- The Kunitz-Type Protein ShPI-1 Inhibits Serine Proteases and Voltage-Gated Potassium Channels.Toxins · 2016Article
- Ectopic expression of a Neospora caninum Kazal type inhibitor triggers developmental defects in Toxoplasma and Plasmodium.PloS one · 2015Article
- Mechanistic insights into mode of actions of novel oligopeptidase B inhibitors for combating leishmaniasis.Journal of molecular modeling · 2014Article
- The oligopeptidase B of Leishmania regulates parasite enolase and immune evasion.The Journal of biological chemistry · 2011Article
- Leishmania infantum chagasi: a genome-based approach to identification of excreted/secreted proteins.Experimental parasitology · 2010Article
- Serine protease activities in Leishmania (Leishmania) chagasi promastigotes.Parasitology research · 2010Article
- Development of protease inhibitors for protozoan infections.Current opinion in infectious diseases · 2008Review
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To investigate the importance of serine proteases in Leishmania amazonensis promastigotes, we analyzed the effects of classical serine protease inhibitors and a Kunitz-type inhibitor, obtained from sea anemone Stichodactyla helianthus (ShPI-I), on the viability and morphology of parasites in culture. Classical inhibitors were selected on the basis of their ability to inhibit L. amazonensis serine proteases, previously described. The N-tosyl-L: -phenylalanine chloromethyl ketone (TPCK) and benzamidine (Bza) inhibitors, which are potential Leishmania proteases inhibitors, in all experimental conditions reduced the parasite viability, with regard to time dependence. On the other hand, N-tosyl-lysine chloromethyl ketone (TLCK) did not significantly affect the parasite viability, as it was poor Leishmania enzymes inhibitor. Ultrastructural analysis demonstrated that both Bza and TPCK induced changes in the flagellar pocket region with membrane alteration, including bleb formation. However, TPCK effects were more pronounced than those of Bza in Leishmania flagellar pocket in plasma membrane, and intracellular vesicular bodies was visualized. ShPI-I proved to be a powerful inhibitor of L. amazonensis serine proteases and the parasite viability. The ultrastructural alterations caused by ShPI-I were more dramatic than those induced by the classical inhibitors. Vesiculation of the flagellar pocket membrane, the appearance of a cytoplasmic vesicle that resembles an autophagic vacuole, and alterations of promastigotes shape resulted.
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