ArticleNucleic acids research2007
Homing endonuclease mediated gene targeting in Anopheles gambiae cells and embryos.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
64 citing papers in PubMed, 146 citations in OpenAlex.
- Understanding the genetics of sex determination in insects and its relevance to genetic pest management.Insect molecular biology · 2025Review
- The haplolethal gene wupA of Drosophila exhibits potential as a target for an X-poisoning gene drive.G3 (Bethesda, Md.) · 2024Article
- Manipulating the Destiny of Wild Populations Using CRISPR.Annual review of genetics · 2023Review
- CRISPR/Cas9-based split homing gene drive targetingProceedings of the National Academy of Sciences of the United States of America · 2023Article
- The Promise and Challenge of Genetic Biocontrol Approaches for Malaria Elimination.Tropical medicine and infectious disease · 2023Review
- Perspectives of vector management in the control and elimination of vector-borne zoonoses.Frontiers in microbiology · 2023Review
- Assessing single-locus CRISPR/Cas9-based gene drive variants in the mosquito Aedes aegypti via single-generation crosses and modeling.G3 (Bethesda, Md.) · 2022Article
- Natural selfish genetic elements should not be defined as gene drives.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Potentials, prospects and applications of genome editing technologies in livestock production.Saudi journal of biological sciences · 2022Review
- Reflection on the Challenges, Accomplishments, and New Frontiers of Gene Drives.Biodesign research · 2022Review
- Manipulating Insect Sex Determination Pathways for Genetic Pest Management: Opportunities and Challenges.Frontiers in bioengineering and biotechnology · 2022Review
- Genetic Approaches for Controlling CRISPR-based Autonomous Homing Gene Drives.Frontiers in bioengineering and biotechnology · 2022Review
- Engineered sex ratio distortion by X-shredding in the global agricultural pest Ceratitis capitata.BMC biology · 2021Article
- Quantifying the risk of vector-borne disease transmission attributable to genetically modified vectors.Royal Society open science · 2021Article
- Making gene drive biodegradable.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Article
- The Potential for a Released Autosomal X-Shredder Becoming a Driving-Y Chromosome and Invasively Suppressing Wild Populations of Malaria Mosquitoes.Frontiers in bioengineering and biotechnology · 2021Article
- Adequacy and sufficiency evaluation of existing EFSA guidelines for the molecular characterisation, environmental risk assessment and post-market environmental monitoring of genetically modified insects containing engineered gene drives.EFSA journal. European Food Safety Authority · 2020Article
- Advances and challenges in genetic technologies to produce single-sex litters.PLoS genetics · 2020Review
- Progress towards engineering gene drives for population control.The Journal of experimental biology · 2020Review
- Review
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Homing endonuclease genes (HEGs) are 'selfish' genetic elements that combine the capability to selectively disrupt specific gene sequences with the ability to rapidly spread from a few individuals to an entire population through homologous recombination repair events. Because of these properties, HEGs are regarded as promising candidates to transfer genetic modifications from engineered laboratory mosquitoes to wild-type populations including Anopheles gambiae the vector of human malaria. Here we show that I-SceI and I-PpoI homing endonucleases cleave their recognition sites with high efficiency in A. gambiae cells and embryos and we demonstrate HEG-induced homologous and non-homologous repair events in a variety of functional assays. We also propose a gene drive system for mosquitoes that is based on our finding that I-PpoI cuts genomic rDNA located on the X chromosome in A. gambiae, which could be used to selectively incapacitate X-carrying spermatozoa thereby imposing a severe male-biased sex ratio.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.