ArticleNucleic acids research2007
Altered target site specificity variants of the I-PpoI His-Cys box homing endonuclease.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Halting Recombinant Adeno-Associated Virus Transgene Expression Using mRNA-Lipid Nanoparticle-Delivered Meganucleases.Human gene therapy · 2025Article
- A unique eukaryotic lineage of composite-like DNA transposons encoding a DDD/E transposase and a His-Me finger homing endonuclease.Mobile DNA · 2022Article
- Pest management by genetic addiction.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- A localized nucleolar DNA damage response facilitates recruitment of the homology-directed repair machinery independent of cell cycle stage.Genes & development · 2015Article
- Linear nicking endonuclease-mediated strand-displacement DNA amplification.Analytical biochemistry · 2011Article
- Comprehensive computational design of mCreI homing endonuclease cleavage specificity for genome engineering.Nucleic acids research · 2011Article
- Successful targeting and disruption of an integrated reporter lentivirus using the engineered homing endonuclease Y2 I-AniI.PloS one · 2011Article
- Evolution of I-SceI homing endonucleases with increased DNA recognition site specificity.Journal of molecular biology · 2011Article
- Pushing the limits of what is achievable in protein-DNA docking: benchmarking HADDOCK's performance.Nucleic acids research · 2010Article
- Sequence conversion by single strand oligonucleotide donors via non-homologous end joining in mammalian cells.The Journal of biological chemistry · 2010Article
- Homing endonucleases: from basics to therapeutic applications.Cellular and molecular life sciences : CMLS · 2010Review
- Cofactor requirement of HpyAV restriction endonuclease.PloS one · 2010Article
- Homing endonucleases catalyze double-stranded DNA breaks and somatic transgene excision in Aedes aegypti.Insect molecular biology · 2009Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
We used a yeast one-hybrid assay to isolate and characterize variants of the eukaryotic homing endonuclease I-PpoI that were able to bind a mutant, cleavage-resistant I-PpoI target or 'homing' site DNA in vivo. Native I-PpoI recognizes and cleaves a semi-palindromic 15-bp target site with high specificity in vivo and in vitro. This target site is present in the 28S or equivalent large subunit rDNA genes of all eukaryotes. I-PpoI variants able to bind mutant target site DNA had from 1 to 8 amino acid substitutions in the DNA-protein interface. Biochemical characterization of these proteins revealed a wide range of site-binding affinities and site discrimination. One-third of variants were able to cleave target site DNA, but there was no systematic relationship between site-binding affinity and site cleavage. Computational modeling of several variants provided mechanistic insight into how amino acid substitutions that contact, or are adjacent to, specific target site DNA base pairs determine I-PpoI site-binding affinity and site discrimination, and may affect cleavage efficiency.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.