ArticleNucleic acids research2007
Identification of eukaryotic promoter regulatory elements using nonhomologous random recombination.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 10 citations in OpenAlex.
- Tailored UPRE2 variants for dynamic gene regulation in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Tracking Effects of SIL1 Increase: Taking a Closer Look Beyond the Consequences of Elevated Expression Level.Molecular neurobiology · 2018Article
- Genome-wide and molecular evolution analysis of the Poplar KT/HAK/KUP potassium transporter gene family.Ecology and evolution · 2012Article
- Feature Identification of Compensatory Gene Pairs without Sequence Homology in Yeast.Comparative and functional genomics · 2012Article
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
Understanding the regulatory logic of a eukaryotic promoter requires the elucidation of the regulatory elements within that promoter. Current experimental or computational methods to discover regulatory motifs within a promoter can be labor intensive and may miss redundant, unprecedented or weakly activating elements. We have developed an unbiased combinatorial approach to rapidly identify new upstream activating sequences (UASs) in a promoter. This approach couples nonhomologous random recombination with an in vivo screen to efficiently identify UASs and does not rely on preconceived hypotheses about promoter regulation or on similarity to known activating sequences. We validated this method using the unfolded protein response (UPR) in yeast and were able to identify both known and potentially novel UASs involved in the UPR. One of the new UASs discovered using this approach implicates Crz1 as a possible activator of Hac1, a transcription factor involved in the UPR. This method has several advantages over existing methods for UAS discovery including its speed, potential generality, sensitivity and lack of false positives and negatives.
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Registered trials
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