ArticleNucleic acids research2007
CTnDOT integrase performs ordered homology-dependent and homology-independent strand exchanges.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 19 citations in OpenAlex.
- The hidden life of integrative and conjugative elements.FEMS microbiology reviews · 2017Review
- Resolution of Mismatched Overlap Holliday Junction Intermediates by the Tyrosine Recombinase IntDOT.Journal of bacteriology · 2017Article
- The Integration and Excision of CTnDOT.Microbiology spectrum · 2015Review
- The Bacteroides thetaiotaomicron protein Bacteroides host factor A participates in integration of the integrative conjugative element CTnDOT into the chromosome.Journal of bacteriology · 2015Article
- Regulation of CTnDOT conjugative transfer is a complex and highly coordinated series of events.mBio · 2013Review
- Article
- IntDOT interactions with core sites during integrative recombination.Journal of bacteriology · 2013Article
- Roles of Exc protein and DNA homology in the CTnDOT excision reaction.Journal of bacteriology · 2012Article
- Resolution of Holliday junction recombination intermediates by wild-type and mutant IntDOT proteins.Journal of bacteriology · 2011Article
- Molecular keys of the tropism of integration of the cholera toxin phage.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Homology-dependent interactions determine the order of strand exchange by IntDOT recombinase.Nucleic acids research · 2010Article
- Challenging a paradigm: the role of DNA homology in tyrosine recombinase reactions.Microbiology and molecular biology reviews : MMBR · 2009Review
- Mutational analysis and homology-based modeling of the IntDOT core-binding domain.Journal of bacteriology · 2009Article
- Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Although the integrase (IntDOT) of the Bacteroides conjugative transposon CTnDOT has been classified as a member of the tyrosine recombinase family, the reaction it catalyzes appears to differ in some features from reactions catalyzed by other tyrosine recombinases. We tested the ability of IntDOT to cleave and ligate activated attDOT substrates in the presence of mismatches. Unlike other tyrosine recombinases, the results revealed that IntDOT is able to perform ligation reactions even when all the bases within the crossover region are mispaired. We also show that there is a strong bias in the order of strand exchanges during integrative recombination. The top strands are exchanged first in reactions that appear to require 2 bp of homology between the partner sites adjacent to the sites of cleavage. The bottom strands are exchanged next in reactions that do not require homology between the partner sites. This mode of coordination of strand exchanges is unique among tyrosine recombinases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.