ArticleGenetics2007
Genetic analysis of the histidine utilization (hut) genes in Pseudomonas fluorescens SBW25.
Article in Genetics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 68 citations in OpenAlex.
- Article
- A GntR family regulator HutC senses PCA to regulate histidine catabolism inMicrobiology spectrum · 2026Article
- Article
- Acid-resistantApplied and environmental microbiology · 2025Article
- Polar accumulation of pyoverdin and exit from stationary phase.microLife · 2024Article
- A New Face of the Old Gene: Deletion of theInternational journal of molecular sciences · 2023Article
- Review
- Obligate cross-feeding expands the metabolic niche of bacteria.Nature ecology & evolution · 2021Article
- Role of a local transcription factor in governing cellular carbon/nitrogen homeostasis in Pseudomonas fluorescens.Nucleic acids research · 2021Article
- Prokaryotic Solute/Sodium Symporters: Versatile Functions and Mechanisms of a Transporter Family.International journal of molecular sciences · 2021Review
- Global Regulatory Roles of the Histidine-Responsive Transcriptional Repressor HutC in Pseudomonas fluorescens SBW25.Journal of bacteriology · 2020Article
- Genotypic and phenotypic analyses reveal distinct population structures and ecotypes for sugar beet-associatedEcology and evolution · 2020Article
- Rewiring the functional complexity between Crc, Hfq and sRNAs to regulate carbon catabolite repression in Pseudomonas.World journal of microbiology & biotechnology · 2019Review
- Repeated Phenotypic Evolution by Different Genetic Routes in Pseudomonas fluorescens SBW25.Molecular biology and evolution · 2019Article
- The absence of SigX results in impaired carbon metabolism and membrane fluidity in Pseudomonas aeruginosa.Scientific reports · 2018Article
- FinR Regulates Expression ofApplied and environmental microbiology · 2018Article
- Characterization of the CrbS/R Two-Component System inFrontiers in microbiology · 2017Article
- Article
- Role of secondary metabolites in the interaction between Pseudomonas fluorescens and soil microorganisms under iron-limited conditions.FEMS microbiology ecology · 2016Article
- Comparative genomics and evolution of transcriptional regulons inMicrobial genomics · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The histidine utilization (hut) locus of Pseudomonas fluorescens SBW25 confers the ability to utilize histidine as a sole carbon and nitrogen source. Genetic analysis using a combination of site-directed mutagenesis and chromosomally integrated lacZ fusions showed the hut locus to be composed of 13 genes organized in 3 transcriptional units: hutF, hutCD, and 10 genes from hutU to hutG (which includes 2 copies of hutH, 1 of which is nonfunctional). Inactivation of hutF eliminated the ability to grow on histidine, indicating that SBW25 degrades histidine by the five-step enzymatic pathway. The 3 hut operons are negatively regulated by the HutC repressor with urocanate (the first intermediate of the histidine degradation pathway) as the physiological inducer. 5'-RACE analysis of transcriptional start sites revealed involvement of both sigma(54) (for the hutU-G operon) and sigma(70) (for hutF); the involvement of sigma(54) was experimentally demonstrated. CbrB (an enhancer binding protein for sigma(54) recruitment) was required for bacterial growth on histidine, indicating positive control of hut gene expression by CbrB. Recognition that a gene (named hutD) encoding a widely distributed conserved hypothetical protein is transcribed along with hutC led to analysis of its role. Mutational and gene fusion studies showed that HutD functions independently of HutC. Growth and fitness assays in laboratory media and on sugar beet seedlings suggest that HutD acts as a governor that sets an upper bound to the level of hut activity.
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