ArticleEukaryotic cell2007
Characterization of metacaspases with trypsin-like activity and their putative role in programmed cell death in the protozoan parasite Leishmania.
Article in Eukaryotic cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 132 citations in OpenAlex.
- Review
- An inclusive assessment of apoptosis mechanisms inCurrent research in parasitology & vector-borne diseases · 2025Review
- Apoptotic proteins in Leishmania donovani: in silico screening, modeling, and validation by knock-out and gene expression analysis.Parasite (Paris, France) · 2025Article
- Arabidopsis metacaspase MC1 localizes in stress granules, clears protein aggregates, and delays senescence.The Plant cell · 2023Article
- Yca1 metacaspase: diverse functions determine how yeast live and let die.FEMS yeast research · 2023Article
- Ecotin: A versatile protease inhibitor of bacteria and eukaryotes.Frontiers in microbiology · 2023Review
- Requirement of Toxoplasma gondii metacaspases for IMC1 maturation, endodyogeny and virulence in mice.Parasites & vectors · 2021Article
- Plasmodium vivax metacaspase 1 (PvMCA1) catalytic domain is conserved in field isolates from Brazilian Amazon.Memorias do Instituto Oswaldo Cruz · 2021Article
- Toxoplasma gondii metacaspase 2 is an important factor that influences bradyzoite formation in the Pru strain.Parasitology research · 2020Article
- Identification and characterization ofBiochemistry and biophysics reports · 2019Article
- Antileishmanial Activity of Niosomal Combination Forms of Tioxolone along with Benzoxonium Chloride against Leishmania tropica.The Korean journal of parasitology · 2019Article
- Cell death in Leishmania.Parasite (Paris, France) · 2019Review
- Metacaspases: Potential Drug Target Against Protozoan Parasites.Frontiers in pharmacology · 2019Review
- Genome-wide identification of barley MCs (metacaspases) and their possible roles in boron-induced programmed cell death.Molecular biology reports · 2018Article
- Zinc depletion promotes apoptosis-like death in drug-sensitive and antimony-resistance Leishmania donovani.Scientific reports · 2017Article
- Methionine aminopeptidase 2 is a key regulator of apoptotic like cell death in Leishmania donovani.Scientific reports · 2017Article
- Metacaspase-binding peptide inhibits heat shock-induced death in Leishmania (L.) amazonensis.Cell death & disease · 2017Article
- Apoptosis-like cell death in Leishmania donovani treated with KalsomeTM10, a new liposomal amphotericin B.PloS one · 2017Article
- Pro-death activity of a BH3 domain in an aquaporin from the protozoan parasite Leishmania.Cell death & disease · 2016Article
- A functional BH3 domain in an aquaporin from Leishmania infantum.Cell death discovery · 2016Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this report, we have characterized two metacaspases of Leishmania donovani, L. donovani metacaspase-1 (LdMC1) and LdMC2. These two proteins show 98% homology with each other, and both contain a characteristic C-terminal proline-rich domain. Both genes are transcribed in promastigotes and axenic amastigotes of L. donovani; however, LdMC1 shows increased mRNA levels in axenic amastigotes. An anti-LdMC antibody was obtained and showed reactivity with a single approximately 42-kDa protein band in both promastigote and axenic amastigote parasite whole-cell lysates by Western blotting. Pulse-chase experiments suggest that LdMCs are not synthesized as proenzymes, and immunofluorescence studies show that LdMCs are associated with the acidocalcisome compartments of L. donovani. Enzymatic assays of immunoprecipitated LdMCs show that native LdMCs efficiently cleave trypsin substrates and are unable to cleave caspase-specific substrates. Consistently, LdMC activity is insensitive to caspase inhibitors and is efficiently inhibited by trypsin inhibitors, such as leupeptin, antipain, and N(alpha)-tosyl-L-lysine-chloromethyl ketone (TLCK). In addition, our results show that LdMC activity was induced in parasites treated with hydrogen peroxide, a known trigger of programmed cell death (PCD) in Leishmania and that parasites overexpressing metacaspases are more sensitive to hydrogen peroxide-induced PCD. These findings suggest that Leishmania metacaspases are not responsible for the caspase-like activities reported in this organism and suggest a possible role for LdMCs as effector molecules in Leishmania PCD.
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Registered trials
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