ArticleEukaryotic cell2007
Tudor nuclease genes and programmed DNA rearrangements in Tetrahymena thermophila.
Article in Eukaryotic cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- Inferring gene-pathway associations from consolidated transcriptome datasets: an interactive gene network explorer forNAR genomics and bioinformatics · 2025Article
- Boundaries of eliminated heterochromatin of Tetrahymena are positioned by the DNA-binding protein Ltl1.Nucleic acids research · 2019Article
- The multifaceted oncogene SND1 in cancer: focus on hepatocellular carcinoma.Hepatoma research · 2018Article
- p100 functions as a metastasis activator and is targeted by tumor suppressing microRNA-320a in lung cancer.Thoracic cancer · 2018Article
- Whats, hows and whys of programmed DNA elimination inOpen biology · 2017Review
- Chromodomain protein Tcd1 is required for macronuclear genome rearrangement and repair in Tetrahymena.Scientific reports · 2015Article
- Tudor staphylococcal nuclease links formation of stress granules and processing bodies with mRNA catabolism in Arabidopsis.The Plant cell · 2015Article
- Tudor Staphylococcal Nuclease plays two antagonistic roles in RNA metabolism under stress.Plant signaling & behavior · 2015Article
- Epigenetics of ciliates.Cold Spring Harbor perspectives in biology · 2013Review
- Sfr13, a member of a large family of asymmetrically localized Sfi1-repeat proteins, is important for basal body separation and stability in Tetrahymena thermophila.Journal of cell science · 2013Article
- Biased transcription and selective degradation of small RNAs shape the pattern of DNA elimination in Tetrahymena.Genes & development · 2012Article
- Tudor staphylococcal nuclease (Tudor-SN) participates in small ribonucleoprotein (snRNP) assembly via interacting with symmetrically dimethylated Sm proteins.The Journal of biological chemistry · 2012Article
- Developmentally programmed, RNA-directed genome rearrangement in Tetrahymena.Development, growth & differentiation · 2012Review
- Genome-Scale Analysis of Programmed DNA Elimination Sites in Tetrahymena thermophila.G3 (Bethesda, Md.) · 2011Article
- RNA interference, growth and differentiation appear normal in African trypanosomes lacking Tudor staphylococcal nuclease.Molecular and biochemical parasitology · 2010Article
- Sequence and structural analysis of 4SNc-Tudor domain protein from Takifugu Rubripes.Bioinformation · 2009Article
- Structural and functional insights into human Tudor-SN, a key component linking RNA interference and editing.Nucleic acids research · 2008Article
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
Proteins containing a Tudor domain and domains homologous to staphylococcal nucleases are found in a number of eukaryotes. These "Tudor nucleases" have been found to be associated with the RNA-induced silencing complex (A. A. Caudy, R. F. Ketting, S. M. Hammond, A. M. Denli, A. M. Bathoorn, B. B. Tops, J. M. Silva, M. M. Myers, G. J. Hannon, and R. H. Plasterk, Nature 425:411-414, 2003). We have identified two Tudor nuclease gene homologs, TTN1 and TTN2, in the ciliate Tetrahymena thermophila, which has two distinct small-RNA pathways. Characterization of single and double KOs of TTN1 and TTN2 shows that neither of these genes is essential for growth or sexual reproduction. Progeny of TTN2 KOs and double knockouts occasionally show minor defects in the small-RNA-guided process of DNA deletion but appear to be normal in hairpin RNA-induced gene silencing, suggesting that Tudor nucleases play only a minor role in RNA interference in Tetrahymena. Previous studies of Tetrahymena have shown that inserted copies of the neo gene from Escherichia coli are often deleted from the developing macronucleus during sexual reproduction (Y. Liu, X. Song, M. A. Gorovsky, and K. M. Karrer, Eukaryot. Cell 4:421-431, 2005; M. C. Yao, P. Fuller, and X. Xi, Science 300:1581-1584, 2003). This transgene deletion phenomenon is hypothesized to be a form of genome defense. Analysis of the Tudor nuclease mutants revealed exceptionally high rates of deletion of the neo transgene at the TTN2 locus but no deletion at the TTN1 locus. When present in the same genome, however, the neo gene is deleted at high rates even at the TTN1 locus, further supporting a role for trans-acting RNA in this process. This deletion is not affected by the presence of the same sequence in the macronucleus, thus providing a counterargument for the role of the macronuclear genome in specifying all sequences for deletion.
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