ArticleEukaryotic cell2007
Two trypanosome-specific proteins are essential factors for 5S rRNA abundance and ribosomal assembly in Trypanosoma brucei.
Article in Eukaryotic cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Role of the RNA-binding protein ZC3H41 in the regulation of ribosomal protein messenger RNAs in trypanosomes.Parasites & vectors · 2023Article
- Trypanosoma brucei L11 Is Essential to Ribosome Biogenesis and Interacts with the Kinetoplastid-Specific Proteins P34 and P37.mSphere · 2019Article
- Article
- The Nuclear Export Receptors TbMex67 and TbMtr2 Are Required for Ribosome Biogenesis in Trypanosoma brucei.mSphere · 2019Article
- Ribosome Assembly in Trypanosomatids: A Novel Therapeutic Target.Trends in parasitology · 2017Article
- Article
- A tRNA methyltransferase paralog is important for ribosome stability and cell division in Trypanosoma brucei.Scientific reports · 2016Article
- Kinetoplastid Specific RNA-Protein Interactions in Trypanosoma cruzi Ribosome Biogenesis.PloS one · 2015Article
- Ribosome biogenesis in african trypanosomes requires conserved and trypanosome-specific factors.Eukaryotic cell · 2014Article
- Global proteomic analysis in trypanosomes reveals unique proteins and conserved cellular processes impacted by arginine methylation.Journal of proteomics · 2013Article
- Ribosome biogenesis requires a highly diverged XRN family 5'->3' exoribonuclease for rRNA processing in Trypanosoma brucei.RNA (New York, N.Y.) · 2013Article
- Association of a novel preribosomal complex in Trypanosoma brucei determined by fluorescence resonance energy transfer.Eukaryotic cell · 2013Article
- Molecular and functional characterization of a Trypanosoma cruzi nuclear adenylate kinase isoform.PLoS neglected tropical diseases · 2013Article
- A novel association between two trypanosome-specific factors and the conserved L5-5S rRNA complex.PloS one · 2012Article
- Characterization of a novel association between two trypanosome-specific proteins and 5S rRNA.PloS one · 2012Article
- Assembly of the Trypanosoma brucei 60S ribosomal subunit nuclear export complex requires trypanosome-specific proteins P34 and P37.Eukaryotic cell · 2009Article
- The RACK1 signal anchor protein from Trypanosoma brucei associates with eukaryotic elongation factor 1A: a role for translational control in cytokinesis.Molecular microbiology · 2008Article
- Trypanosoma brucei RNA binding proteins p34 and p37 mediate NOPP44/46 cellular localization via the exportin 1 nuclear export pathway.Eukaryotic cell · 2007Article
- Article
- Eukaryotic 5S rRNA biogenesis.Wiley interdisciplinary reviews. RNAReview
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6 authors at 1 institution in 1 country.
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Abstract
We have previously identified and characterized two novel nuclear RNA binding proteins, p34 and p37, which have been shown to bind 5S rRNA in Trypanosoma brucei. These two proteins are nearly identical, with one major difference, an 18-amino-acid insert in the N-terminal region of p37, as well as three minor single-amino-acid differences. Homologues to p34 and p37 have been found only in other trypanosomatids, suggesting that these proteins are unique to this ancient family. We have employed RNA interference (RNAi) studies in order to gain further insight into the interaction between p34 and p37 with 5S rRNA in T. brucei. In our p34/p37 RNAi cells, decreased expression of the p34 and p37 proteins led to morphological alterations, including loss of cell shape and vacuolation, as well as to growth arrest and ultimately to cell death. Disruption of a higher-molecular-weight complex containing 5S rRNA occurs as well as a dramatic decrease in 5S rRNA levels, suggesting that p34 and p37 serve to stabilize 5S rRNA. In addition, an accumulation of 60S ribosomal subunits was observed, accompanied by a significant decrease in overall protein synthesis within p34/p37 RNAi cells. Thus, the loss of the trypanosomatid-specific proteins p34 and p37 correlates with a diminution in 5S rRNA levels as well as a decrease in ribosome activity and an alteration in ribosome biogenesis.
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