ArticleClinical and vaccine immunology : CVI2007
Molecular characterization of clinical isolates of Mycobacterium tuberculosis and their association with phenotypic virulence in human macrophages.
Article in Clinical and vaccine immunology : CVI, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- Effects ofAnnals of translational medicine · 2022Article
- Genetically RelatedVeterinary sciences · 2019Article
- Cytokine Biomarkers Associated with Human Extra-Pulmonary Tuberculosis Clinical Strains and Symptoms.Frontiers in microbiology · 2018Article
- Virulence ofFrontiers in cellular and infection microbiology · 2018Article
- Modeling tuberculosis pathogenesis through ex vivo lung tissue infection.Tuberculosis (Edinburgh, Scotland) · 2017Article
- Whole Genome Sequencing of Mycobacterium tuberculosis Isolates From Extrapulmonary Sites.Omics : a journal of integrative biology · 2017Article
- Comparative Whole-Genomic Analysis of an Ancient L2 LineageFrontiers in cellular and infection microbiology · 2017Article
- Article
- Association between spoligotype-VNTR types and virulence of Mycobacterium bovis in cattle.Virulence · 2014Article
- Mycobacterium tuberculosis lineage--what's in your lungs?Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2012Article
- Article
- Non-Beijing strains of Mycobacterium tuberculosis in China.Journal of clinical microbiology · 2011Article
- Article
- Presence of RD149 deletions in M. tuberculosis Central Asian Strain 1 isolates affect growth and TNFα induction in THP-1 monocytes.PloS one · 2011Article
- Characterization of Mycobacterium tuberculosis Beijing isolates from the Mediterranean area.BMC microbiology · 2010Article
- Impaired activation of Stat1 and c-Jun as a possible defect in macrophages of patients with active tuberculosis.Clinical and experimental immunology · 2009Article
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Among 125 clinical isolates of Mycobacterium tuberculosis collected in Hong Kong and Shanghai, China, between 2002 and 2004, IS6110 typing revealed that 71 strains (57%) belonged to the Beijing family. The intracellular growth of the strains in human peripheral blood monocyte-derived macrophages was measured ex vivo on days 0, 3, 6, and 10. Among all tested strains, three hypervirulent strains showed significant increases in intracellular growth after 10 days of incubation. With an initial bacterial load of 10(4) CFU, most of the clinical isolates and H37Ra (an avirulent strain) exhibited no intracellular survival on day 10, while the three hypervirulent strains together with H37Rv (a virulent strain) showed on average a two- to fourfold rise in CFU count. These three hypervirulent strains belonging to a non-Beijing family were isolated from patients suffering from tuberculosis meningitis. Cytokines secreted by gamma interferon-activated macrophages were measured daily after challenge with selected strains of M. tuberculosis. The levels of tumor necrosis factor alpha were elevated after 24 h of infection among all strains, but the levels were significantly lower among the three hypervirulent strains, whereas interleukin 10 (IL-10) and IL-12 were not detected. Results were concordant with the differential expression of the corresponding cytokine genes in activated macrophages, as monitored by real-time PCR. Our findings highlighted that these three hypervirulent strains may possess an innate mechanism for escaping host immunity, which accounts for their characteristic virulence in patients presenting with a more severe form of disease.
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