ArticleNucleic acids research2007
Direct immobilization of DNA oligomers onto the amine-functionalized glass surface for DNA microarray fabrication through the activation-free reaction of oxanine.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Urinary Cell-Free DNA detection platform for bacterial cystitis diagnosis via spot-based machine learning.Journal of advanced research · 2026Article
- Stable and programmable formation of DNA nanostructures via inter-strand crosslinking by assembly-triggered oxanine and amine linkage.Nucleic acids research · 2026Article
- Development of a clinical microarray system for genetic analysis screening.Practical laboratory medicine · 2023Article
- Novel enzymatic single-nucleotide modification of DNA oligomer: prevention of incessant incorporation of nucleotidyl transferase by ribonucleotide-borate complex.Nucleic acids research · 2019Article
- Microbiological Sensing Technologies: A Review.Bioengineering (Basel, Switzerland) · 2018Review
- Investigation and development of quantum dot-encoded microsphere bioconjugates for DNA detection by flow cytometry.Journal of fluorescence · 2012Article
- T7 RNA polymerases backed up by covalently trapped proteins catalyze highly error prone transcription.The Journal of biological chemistry · 2012Article
- Characterization of surface modification on self-assembled monolayer-based piezoelectric crystal immunosensor for the quantification of serum α-fetoprotein.Journal of materials science. Materials in medicine · 2011Article
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Authors and funding
9 authors.
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Abstract
Oxanine having an O-acylisourea structure was explored to see if its reactivity with amino group is useful in DNA microarray fabrication. By the chemical synthesis, a nucleotide unit of oxanine (Oxa-N) was incorporated into the 5'-end of probe DNA with or without the -(CH2)n- spacers (n = 3 and 12) and found to immobilize the probe DNA covalently onto the NH2-functionalized glass slide by one-pot reaction, producing the high efficiency of the target hybridization. The methylene spacer, particularly the longer one, generated higher efficiency of the target recognition although there was little effect on the amount of the immobilized DNA oligomers. The post-spotting treatment was also carried out under the mild conditions (at 25 or 42 degrees C) and the efficiencies of the immobilization and the target recognition were evaluated similarly, and analogous trends were obtained. It has also been determined under the mild conditions that the humidity and time of the post-spotting treatment, pH of the spotting solution and the synergistic effects with UV-irradiation largely contribute to the desired immobilization and resulting target recognition. Immobilization of DNA oligomer by use of Oxa-N on the NH2-functionalized surface without any activation step would be employed as one of the advanced methods for generating DNA-conjugated solid surface.
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