ArticleProceedings of the National Academy of Sciences of the United States of America2007
Protection against telomeric position effects by the chicken cHS4 beta-globin insulator.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 32 citations in OpenAlex.
- Analog epigenetic memory revealed by targeted chromatin editing.Cell genomics · 2025Article
- The sound of silence: Transgene silencing in mammalian cell engineering.Cell systems · 2022Review
- Improved efficiency of genome editing by constitutive expression of Cas9 endonuclease in genetically-modified mice.3 Biotech · 2021Article
- Shortened nuclear matrix attachment regions are sufficient for replication and maintenance of episomes in mammalian cells.Molecular biology of the cell · 2019Article
- Article
- Bring It to an End: Does Telomeres Size Matter?Cells · 2019Review
- A chimeric HS4 insulator-scaffold attachment region enhances transgene expression in transfected Chinese hamster ovary cells.FEBS open bio · 2017Article
- Identification of regulatory motifs in the CHO genome for stable monoclonal antibody production.Cytotechnology · 2017Article
- An insulator element located at the cyclin B1 interacting protein 1 gene locus is highly conserved among mammalian species.PloS one · 2015Article
- A chimeric HS4-SAR insulator (IS2) that prevents silencing and enhances expression of lentiviral vectors in pluripotent stem cells.PloS one · 2014Article
- Epigenetic regulatory elements associate with specific histone modifications to prevent silencing of telomeric genes.Nucleic acids research · 2014Article
- Chicken β-globin insulators fail to shield the nkx2.5 promoter from integration site effects in zebrafish.Molecular genetics and genomics : MGG · 2013Article
- Telomere length homeostasis and telomere position effect on a linear human artificial chromosome are dictated by the genetic background.Nucleic acids research · 2012Article
- Prototypic chromatin insulator cHS4 protects retroviral transgene from silencing in Schistosoma mansoni.Transgenic research · 2012Article
- Developing genetically engineered mouse models to study tumor suppression.Current protocols in mouse biology · 2012Article
- Genome-wide profiling of forum domains in Drosophila melanogaster.Nucleic acids research · 2011Article
- A tissue-specific, activation-inducible, lentiviral vector regulated by human CD40L proximal promoter sequences.Gene therapy · 2011Article
- An insulator embedded in the chicken α-globin locus regulates chromatin domain configuration and differential gene expression.Nucleic acids research · 2011Article
- Synaptonemal complex stability depends on repressive histone marks of the lateral element-associated repeat sequences.Chromosoma · 2010Article
- Transcription factor CTF1 acts as a chromatin domain boundary that shields human telomeric genes from silencing.Molecular and cellular biology · 2009Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Epigenetic silencing of genes relocated near telomeres, termed telomeric position effect, has been extensively studied in yeast and more recently in vertebrates. However, protection of a transgene against telomeric position effects by chromatin insulators has not yet been addressed. In this work we investigated the capacity of the chicken beta-globin insulator cHS4 to shield a transgene against silencing by telomeric heterochromatin. Using telomeric repeats, we targeted transgene integration into telomeres of the chicken cell line HD3. When the chicken cHS4 insulator is incorporated to the transgene, we observe a sustained gene expression of single-copy integrants that can be maintained for >100 days of continuous culture. However, uninsulated single-copy clones showed an accelerated gene expression extinction profile. Unexpectedly, telomeric silencing was not reversed with trichostatin A or nicotidamine. In contrast, significant reactivation was obtained with 5-aza-2'-deoxycytidine, consistent with the subtelomeric DNA methylation status. Strikingly, insulated transgenes integrated into telomeric regions were enriched in histone methylation, such as H3K4me2 and H3K79me2, but not in histone acetylation. Furthermore, the cHS4 insulator counteracts telomeric position effects in an upstream stimulatory factor-independent manner. Our results suggest that this insulator has the capacity to adapt to different chromatin propagation signals in distinct insertional epigenome environments.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.