ArticleJournal of neuroscience methods2007
Matching native electrical stimulation by graded chemical stimulation in isolated mouse adrenal chromaffin cells.
Article in Journal of neuroscience methods, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
18 citing papers in PubMed, 23 citations in OpenAlex.
- Does Monopolar Electrocautery Affect the Fetus during Cesarean Section?Medicina (Kaunas, Lithuania) · 2024Article
- Synaptotagmin-7 enhances calcium-sensing of chromaffin cell granules and slows discharge of granule cargos.Journal of neurochemistry · 2020Article
- The high-affinity calcium sensor synaptotagmin-7 serves multiple roles in regulated exocytosis.The Journal of general physiology · 2018Review
- Synaptotagmin isoforms confer distinct activation kinetics and dynamics to chromaffin cell granules.The Journal of general physiology · 2017Article
- An interplay between the serotonin transporter (SERT) and 5-HT receptors controls stimulus-secretion coupling in sympathoadrenal chromaffin cells.Neuropharmacology · 2016Article
- Distinct fusion properties of synaptotagmin-1 and synaptotagmin-7 bearing dense core granules.Molecular biology of the cell · 2014Article
- Syndapin 3 modulates fusion pore expansion in mouse neuroendocrine chromaffin cells.American journal of physiology. Cell physiology · 2014Article
- Article
- Influence of Fimasartan (a Novel AT(1) Receptor Blocker) on Catecholamine Release in the Adrenal Medulla of Spontaneously Hypertensive Rats.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2013Article
- Functional chromaffin cell plasticity in response to stress: focus on nicotinic, gap junction, and voltage-gated Ca2+ channels.Journal of molecular neuroscience : MN · 2012Review
- Activity-dependent fusion pore expansion regulated by a calcineurin-dependent dynamin-syndapin pathway in mouse adrenal chromaffin cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Gabapentin inhibits catecholamine release from adrenal chromaffin cells.Anesthesiology · 2012Article
- A Microfluidic Approach to Pulsatile Delivery of Drugs for Neurobiological Studies.Journal of microelectromechanical systems : a joint IEEE and ASME publication on microstructures, microactuators, microsensors, and microsystems · 2012Article
- Dynamin and myosin regulate differential exocytosis from mouse adrenal chromaffin cells.Cellular and molecular neurobiology · 2010Review
- Revisiting the stimulus-secretion coupling in the adrenal medulla: role of gap junction-mediated intercellular communication.Molecular neurobiology · 2009Review
- Cortical F-actin, the exocytic mode, and neuropeptide release in mouse chromaffin cells is regulated by myristoylated alanine-rich C-kinase substrate and myosin II.Molecular biology of the cell · 2009Article
- Dynamin I plays dual roles in the activity-dependent shift in exocytic mode in mouse adrenal chromaffin cells.Archives of biochemistry and biophysics · 2008Article
- Myosin II activation and actin reorganization regulate the mode of quantal exocytosis in mouse adrenal chromaffin cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Adrenal chromaffin cells release multiple transmitters in response to sympathetic stimulation. Modest cell firing, matching sympathetic tone, releases small freely soluble catecholamines. Elevated electrical firing rates matching input under sympathetic stress results in release of catecholamines as well as semi-soluble vaso- and neuro-active peptides packaged within the dense core of the secretory granule. This activity-dependent differential transmitter release has been shown to rely on a mechanistic shift in the mode of exocytosis through the regulated dilation of the secretory fusion pore between granule and cell surface membranes. However, biochemical description of the mechanism regulating fusion pore dilation remains elusive. In the experimental setting, electrical stimulation designed to mimic sympathetic input, is achieved through single-cell voltage-clamp. While precise, this approach is incompatible with biochemical and proteomic analysis, both of which require large sample sizes. We address this limitation in the current study. We describe a bulk chemical stimulation paradigm calibrated to match defined electrical activity. We utilize calcium and single-cell amperometric measurements to match extracellular potassium concentrations to physiological electrical stimulation under sympathetic tone as well as acute stress conditions. This approach provides larger samples of uniformly stimulated cells for determining molecular players in activity-dependent differential transmitter release from adrenal chromaffin cells.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.