ArticlePharmaceutical research2008
Genetically engineered block copolymers: influence of the length and structure of the coiled-coil blocks on hydrogel self-assembly.
Article in Pharmaceutical research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Molecular Engineering of Recombinant Protein Hydrogels: Programmable Design and Biomedical Applications.Gels (Basel, Switzerland) · 2025Review
- Engineered Protein Hydrogels as Biomimetic Cellular Scaffolds.Advanced materials (Deerfield Beach, Fla.) · 2024Review
- Hydrophilic biomaterials: From crosslinked and self-assembled hydrogels to polymer-drug conjugates and drug-free macromolecular therapeutics.Journal of controlled release : official journal of the Controlled Release Society · 2024Article
- Microrheology for biomaterial design.APL bioengineering · 2020Review
- Coiled-Coils: the Molecular Zippers that Self-Assemble Protein Nanostructures.International journal of molecular sciences · 2020Review
- Protein-Engineered Functional Materials.Advanced healthcare materials · 2019Review
- Injectable tissue integrating networks from recombinant polypeptides with tunable order.Nature materials · 2018Article
- Author Correction: Injectable tissue integrating networks from recombinant polypeptides with tunable order.Nature materials · 2018Article
- Scalable manufacturing of biomimetic moldable hydrogels for industrial applications.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Hierarchical cascades of instability govern the mechanics of coiled coils: helix unfolding precedes coil unzipping.Biophysical journal · 2014Article
- A quantitative recipe for engineering protein polymer nanoparticles.Polymer chemistry · 2014Article
- Multiple site-selective insertions of noncanonical amino acids into sequence-repetitive polypeptides.Chembiochem : a European journal of chemical biology · 2013Article
- Reinforcement of Shear Thinning Protein Hydrogels by Responsive Block Copolymer Self-Assembly.Advanced functional materials · 2013Article
- Smart self-assembled hybrid hydrogel biomaterials.Angewandte Chemie (International ed. in English) · 2012Review
- Protein-based block copolymers.Biomacromolecules · 2011Review
- Yielding Behavior in Injectable Hydrogels from Telechelic Proteins.Macromolecules · 2010Article
- Rheological properties of peptide-based hydrogels for biomedical and other applications.Chemical Society reviews · 2010Review
- PROTEIN TEMPLATES IN HARD TISSUE ENGINEERING.Nano today · 2010Article
- HYDROGELS FROM SOFT CONTACT LENSES AND IMPLANTS TO SELF-ASSEMBLED NANOMATERIALS.Journal of polymer science. Part A, Polymer chemistry · 2009Article
- Stimuli-responsive properties of peptide-based copolymers studied via directional growth of self-assembled patterns on solid substrate.Biomacromolecules · 2009Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
purposeTo explore the relationship between the structure of block polypeptides and their self-assembly into hydrogels. To investigate structural parameters that influence hydrogel formation and physical properties.
methodsThree ABA triblock and two AB diblock coiled-coil containing polypeptides were designed and biologically synthesized. The triblock polypeptides had two terminal coiled-coil (A) domains and a central random coil (B) segment. The coiled-coil domains were different in their lengths, and tyrosine residues were incorporated at selected solvent-exposed positions in order to increase the overall hydrophobicity of the coiled-coil domains. The secondary structures of these polypeptides were characterized by circular dichroism and analytical ultracentrifugation. The formation of hydrogel structures was evaluated by microrheology and scanning electron microscopy.
resultsHydrogels self-assembled from the triblock polypeptides, and had interconnected network microstructures. Hydrogel formation was reversible. Denaturation of coiled-coil domains by guanidine hydrochloride (GdnHCl) resulted in disassembly of the hydrogels. Removal of GdnHCl by dialysis caused coiled-coil refolding and hydrogel reassembly.
conclusionsProtein ABA triblock polypeptides composed of a central random block flanked by two coiled-coil forming sequences self-assembled into hydrogels. Hydrogel formation and physical properties may be manipulated by choosing the structure and changing the length of the coiled-coil blocks. These self-assembling systems have a potential as in-situ forming depots for protein delivery.
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Registered trials
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