ArticlePlant cell reports2008
Genetic transformation of sweet orange with the coat protein gene of Citrus psorosis virus and evaluation of resistance against the virus.
Article in Plant cell reports, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 64 citations in OpenAlex.
- Glycerophosphodiester phosphodiesterase 1 mediates G3P accumulation for Eureka lemon resistance to citrus yellow vein clearing virus.Horticulture research · 2025Article
- Citrus Psorosis Virus: Current Insights on a Still Poorly Understood Ophiovirus.Microorganisms · 2020Review
- Transgenic Sweet Orange expressing hairpin CP-mRNA in the interstock confers tolerance to citrus psorosis virus in the non-transgenic scion.Transgenic research · 2020Article
- New Plant Breeding Techniques in Citrus for the Improvement of Important Agronomic Traits. A Review.Frontiers in plant science · 2020Review
- Article
- Overexpression of a thaumatin-like protein gene from Vitis amurensis improves downy mildew resistance in Vitis vinifera grapevine.Protoplasma · 2017Article
- Small RNA Based Genetic Engineering for Plant Viral Resistance: Application in Crop Protection.Frontiers in microbiology · 2017Review
- Early-flowering sweet orange mutant 'x11' as a model for functional genomic studies of Citrus.BMC research notes · 2014Article
- Recent advances in Citrus psorosis virus.Virusdisease · 2014Review
- Temporal and spatial control of gene expression in horticultural crops.Horticulture research · 2014Review
- Genetic transformation in citrus.TheScientificWorldJournal · 2013Review
- GUS expression in sweet oranges (Citrus sinensis L. Osbeck) driven by three different phloem-specific promoters.Plant cell reports · 2012Article
- Functional analysis and expression profiling of HcrVf1 and HcrVf2 for development of scab resistant cisgenic and intragenic apples.Plant molecular biology · 2011Article
- Differential resistance to Citrus psorosis virus in transgenic Nicotiana benthamiana plants expressing hairpin RNA derived from the coat protein and 54K protein genes.Plant cell reports · 2009Article
- Transgenic peppers that are highly tolerant to a new CMV pathotype.Plant cell reports · 2009Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Citrus psorosis is a serious viral disease affecting citrus trees in many countries. Its causal agent is Citrus psorosis virus (CPsV), the type member of genus Ophiovirus. CPsV infects most important citrus varieties, including oranges, mandarins and grapefruits, as well as hybrids and citrus relatives used as rootstocks. Certification programs have not been sufficient to control the disease and no sources of natural resistance have been found. Pathogen-derived resistance (PDR) can provide an efficient alternative to control viral diseases in their hosts. For this purpose, we have produced 21 independent lines of sweet orange expressing the coat protein gene of CPsV and five of them were challenged with the homologous CPV 4 isolate. Two different viral loads were evaluated to challenge the transgenic plants, but so far, no resistance or tolerance has been found in any line after 1 year of observations. In contrast, after inoculation all lines showed characteristic symptoms of psorosis in the greenhouse. The transgenic lines expressed low and variable amounts of the cp gene and no correlation was found between copy number and transgene expression. One line contained three copies of the cp gene, expressed low amounts of the mRNA and no coat protein. The ORF was cytosine methylated suggesting a PTGS mechanism, although the transformant failed to protect against the viral load used. Possible causes for the failed protection against the CPsV are discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.