ArticlePloS one2007
Evidence for a minimal eukaryotic phosphoproteome?
Article in PloS one, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Age related structural and functional changes in left ventricular performance in healthy subjects: a 2D echocardiographic study.The international journal of cardiovascular imaging · 2019Article
- Improved intra-array and interarray normalization of peptide microarray phosphorylation for phosphorylome and kinome profiling by rational selection of relevant spots.Scientific reports · 2016Article
- RF-Phos: A Novel General Phosphorylation Site Prediction Tool Based on Random Forest.BioMed research international · 2016Article
- Kinotypes: stable species- and individual-specific profiles of cellular kinase activity.BMC genomics · 2013Article
- A highly scalable peptide-based assay system for proteomics.PloS one · 2012Article
- Kinome profiling.Scientifica · 2012Review
- Deciphering enzyme function using peptide arrays.Molecular biotechnology · 2011Review
- Major remodelling of the murine stem cell kinome following differentiation in the hematopoietic compartment.Journal of proteome research · 2011Article
- Kinase activity profiling of gram-negative pneumonia.Molecular medicine (Cambridge, Mass.) · 2011Article
- Article
- A novel p53 phosphorylation site within the MDM2 ubiquitination signal: I. phosphorylation at SER269 in vivo is linked to inactivation of p53 function.The Journal of biological chemistry · 2010Article
- Kinome profiling of sugar signaling in plants using multiple platforms.Plant signaling & behavior · 2009Article
- Article
- Comparison of peptide array substrate phosphorylation of c-Raf and mitogen activated protein kinase kinase kinase 8.PloS one · 2009Article
- A site-specific, multiplexed kinase activity assay using stable-isotope dilution and high-resolution mass spectrometry.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Comparative phosphoproteomics reveals evolutionary and functional conservation of phosphorylation across eukaryotes.Genome biology · 2008Article
- Large-scale phosphorylation mapping reveals the extent of tyrosine phosphorylation in Arabidopsis.Molecular systems biology · 2008Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundReversible phosphorylation catalysed by kinases is probably the most important regulatory mechanism in eukaryotes. METHODOLOGY/PRINCIPAL
findingsWe studied the in vitro phosphorylation of peptide arrays exhibiting the majority of PhosphoBase-deposited protein sequences, by factors in cell lysates from representatives of various branches of the eukaryotic species. We derived a set of substrates from the PhosphoBase whose phosphorylation by cellular extracts is common to the divergent members of different kingdoms and thus may be considered a minimal eukaryotic phosphoproteome. The protein kinases (or kinome) responsible for phosphorylation of these substrates are involved in a variety of processes such as transcription, translation, and cytoskeletal reorganisation. CONCLUSIONS/SIGNIFICANCE: These results indicate that the divergence in eukaryotic kinases is not reflected at the level of substrate phosphorylation, revealing the presence of a limited common substrate space for kinases in eukaryotes and suggests the presence of a set of kinase substrates and regulatory mechanisms in an ancestral eukaryote that has since remained constant in eukaryotic life.
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