ArticleMolecular imaging
Fluorescence lifetime imaging system for in vivo studies.
Article in Molecular imaging. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 99 citations in OpenAlex.
- The Role of Tumor pH in Breast Cancer Imaging: Biology, Diagnostic Applications, and Emerging Techniques.Diagnostics (Basel, Switzerland) · 2025Review
- Nano- and Microsensors for In Vivo Real-Time Electrochemical Analysis: Present and Future Perspectives.Nanomaterials (Basel, Switzerland) · 2022Review
- Renal pH Mapping Using Chemical Exchange Saturation Transfer (CEST) MRI: Experimental Protocol.Methods in molecular biology (Clifton, N.J.) · 2021Article
- A New Class of Smart Gadolinium Contrast Agent for Tissue pH Probing Using Magnetic Resonance Imaging.Molecules (Basel, Switzerland) · 2020Article
- Cardiac pH-Imaging With Hyperpolarized MRI.Frontiers in cardiovascular medicine · 2020Review
- Review
- Using in vivo fluorescence lifetime imaging to detect HER2-positive tumors.EJNMMI research · 2018Article
- Extracellular pH is a biomarker enabling detection of breast cancer and liver cancer using CEST MRI.Oncotarget · 2017Article
- Imaging of pH in vivo using hyperpolarizedNature communications · 2017Article
- Gear Up for a pH Shift: A Responsive Iron(II) 2-Amino-6-picolyl-Appended Macrocyclic paraCEST Agent That Protonates at a Pendent Group.Inorganic chemistry · 2016Article
- Endothelial HIF signaling regulates pulmonary fibrosis-associated pulmonary hypertension.American journal of physiology. Lung cellular and molecular physiology · 2016Article
- Macroscopic-imaging technique for subsurface quantification of near-infrared markers during surgery.Journal of biomedical optics · 2015Article
- Evaluating pH in the Extracellular Tumor Microenvironment Using CEST MRI and Other Imaging Methods.Advances in radiology · 2015Article
- In vivo assessment of HER2 receptor density in HER2-positive tumors by near-infrared imaging, using repeated injections of the fluorescent probe.Technology in cancer research & treatment · 2014Article
- Reduced temporal sampling effect on accuracy of time-domain fluorescence lifetime Förster resonance energy transfer.Journal of biomedical optics · 2014Article
- Re-evaluation of biotin-streptavidin conjugation in Förster resonance energy transfer applications.Journal of biomedical optics · 2014Article
- In vivo fluorescence lifetime imaging for monitoring the efficacy of the cancer treatment.Clinical cancer research : an official journal of the American Association for Cancer Research · 2014Article
- In vivo tumour extracellular pH monitoring using electron paramagnetic resonance: the effect of X-ray irradiation.NMR in biomedicine · 2014Article
- Fluorescence-lifetime molecular imaging can detect invisible peritoneal ovarian tumors in bloody ascites.Cancer science · 2014Article
- Spatial light modulator based active wide-field illumination for ex vivo and in vivo quantitative NIR FRET imaging.Biomedical optics express · 2014Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
In this article, a fluorescence lifetime imaging system for small animals is presented. Data were collected by scanning a region of interest with a measurement head, a linear fiber array with fixed separations between a single source fiber and several detection fibers. The goal was to localize tumors and monitor their progression using specific fluorescent markers. We chose a near-infrared contrast agent, Alexa Fluor 750 (Invitrogen Corp., Carlsbad, CA). Preliminary results show that the fluorescence lifetime for this dye was sensitive to the immediate environment of the fluorophore (in particular, pH), making it a promising candidate for reporting physiologic changes around a fluorophore. To quantify the intrinsic lifetime of deeply embedded fluorophores, we performed phantom experiments to investigate the contribution of photon migration effects on observed lifetime by calculating the fluorescence intensity decay time. A previously proposed theoretical model of migration, based on random walk theory, is also substantiated by new experimental data. The developed experimental system has been used for in vivo mouse imaging with Alexa Fluor 750 contrast agent conjugated to tumor-specific antibodies (trastuzumab [Herceptin]). Three-dimensional mapping of the fluorescence lifetime indicates lower lifetime values in superficial breast cancer tumors in mice.
Indexed as
Identifiers
17711778PMC3512197W2108166331What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.