ArticleTransgenic research2008
Visualisation of plastids in endosperm, pollen and roots of transgenic wheat expressing modified GFP fused to transit peptides from wheat SSU RubisCO, rice FtsZ and maize ferredoxin III proteins.
Article in Transgenic research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- A Simplified Wheat Protoplast Transformation System and Guideline for Avoiding Protein Localization Artifacts.Plants (Basel, Switzerland) · 2026Article
- High-efficiency leucoplast transit peptides for manipulating plastid protein production.Nature plants · 2025Article
- Applications of CPPs in Genome Editing of Plants.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Fluorescent Labeling and Confocal Microcopy of Plastids and Stromules.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Tissue-Specific Regulation of Plastid Protein Import via Transit-Peptide Motifs.The Plant cell · 2020Article
- Developmental regulation of protein import into plastids.Photosynthesis research · 2018Review
- Visualisation of plastid degradation in sperm cells of wheat pollen.Protoplasma · 2017Article
- The first crop plant genetically engineered to release an insect pheromone for defence.Scientific reports · 2015Article
- Protein import into isolated pea root leucoplasts.Frontiers in plant science · 2015Article
- Fluorescent Protein Aided Insights on Plastids and their Extensions: A Critical Appraisal.Frontiers in plant science · 2015Review
- Delivering sustainable crop protection systems via the seed: exploiting natural constitutive and inducible defence pathways.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- Border control: selectivity of chloroplast protein import and regulation at the TOC-complex.Frontiers in plant science · 2014Review
- An appropriate concentration of arginine is required for normal root growth in rice.Plant signaling & behavior · 2014Article
- Expression of Brassica oleracea FtsZ1-1 and MinD alters chloroplast division in Nicotiana tabacum generating macro- and mini-chloroplasts.Plant cell reports · 2012Article
- Differential age-dependent import regulation by signal peptides.PLoS biology · 2012Article
- Visualisation of stromules in transgenic wheat expressing a plastid-targeted yellow fluorescent protein.Planta · 2011Article
- Dynamic morphologies of pollen plastids visualised by vegetative-specific FtsZ1-GFP in Arabidopsis thaliana.Protoplasma · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The ability to target marker proteins to specific subcellular compartments is a powerful research tool to study the structure and development of organelles. Here transit sequences from nuclear-encoded, plastid proteins, namely rice FtsZ, maize non-photosynthetic ferredoxin III (FdIII) and the small subunit of RubisCO were used to target a modified synthetic GFP (S65G, S72A) to plastids. The localisations of the fusion proteins expressed in transgenic wheat plants and under the control of the rice actin promoter were compared to an untargeted GFP control. GFP fluorescence was localised to non-green plastids in pollen, roots and seed endosperm and detected in isolated leaf chloroplasts using a GFP-specific antibody. Transit peptides appeared to influence the relative fluorescence intensities of plastids in different tissues. This is consistent with differential targeting and/or turnover of GFP fusion proteins in different plastid types. Replacement of GFP sequences with alternative coding regions enables immediate applications of our vectors for academic research and commercial applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.