ArticlePlant cell reports2007
Evidence of artemisinin production from IPP stemming from both the mevalonate and the nonmevalonate pathways.
Article in Plant cell reports, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 175 citations in OpenAlex.
- Anticoccidial effects of Artemisia annua ethanolic extract: prevention, simultaneous challenge-medication, and treatment.Parasitology research · 2017Trial
- AaSnRK2.6 interacts with AaORA to positively regulate artemisinin biosynthesis inHorticulture research · 2026Article
- Unlocking the potential of3 Biotech · 2025Review
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- Genetic analysis reveals the inconsistency of amorpha-4,11-diene synthase, a key enzyme in the artemisinin synthesis pathway, in asteraceae.Chinese medicine · 2023Article
- Article
- Review
- Jasmonate- and abscisic acid-activated AaGSW1-AaTCP15/AaORA transcriptional cascade promotes artemisinin biosynthesis in Artemisia annua.Plant biotechnology journal · 2021Article
- Molecular Cloning and Functional Analysis of 1-Deoxy-D-Xylulose 5-Phosphate Reductoisomerase fromGenes · 2021Article
- Isotope ratio-based quantification of carbon assimilation highlights the role of plastidial isoprenoid precursor availability in photosynthesis.Plant methods · 2021Article
- Chromatin Accessibility Is Associated with Artemisinin Biosynthesis Regulation inMolecules (Basel, Switzerland) · 2021Article
- Transcriptomic profiling reveals MEP pathway contributing to ginsenoside biosynthesis in Panax ginseng.BMC genomics · 2019Article
- Isoprenyl diphosphate synthases: the chain length determining step in terpene biosynthesis.Planta · 2019Review
- The YABBY Family Transcription Factor AaYABBY5 Directly Targets Cytochrome P450 Monooxygenase (CYP71AV1) and Double-Bond Reductase 2 (DBR2) Involved in Artemisinin Biosynthesis inFrontiers in plant science · 2019Article
- Jasmonate promotes artemisinin biosynthesis by activating the TCP14-ORA complex inScience advances · 2018Article
- An investigation of the3 Biotech · 2018Review
- Production of Plant Secondary Metabolites: Examples, Tips and Suggestions for Biotechnologists.Genes · 2018Review
- Molecular Characterization of theFrontiers in plant science · 2018Article
- Volatile terpenoids: multiple functions, biosynthesis, modulation and manipulation by genetic engineering.Planta · 2017Review
- Effects of different doses of cadmium on secondary metabolites and gene expression in Artemisia annua L.Frontiers of medicine · 2017Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The potent antimalarial sesquiterpene lactone, artemisinin, is produced in low quantities by the plant Artemisia annua L. The source and regulation of the isopentenyl diphosphate (IPP) used in the biosynthesis of artemisinin has not been completely characterized. Terpenoid biosynthesis occurs in plants via two IPP-generating pathways: the mevalonate pathway in the cytosol, and the non-mevalonate pathway in plastids. Using inhibitors specific to each pathway, it is possible to resolve which supplies the IPP precursor to the end product. Here, we show the effects of inhibition on the two pathways leading to IPP for artemisinin production in plants. We grew young (7-14 days post cotyledon) plants in liquid culture, and added mevinolin to the medium to inhibit the mevalonate pathway, or fosmidomycin to inhibit the non-mevalonate pathway. Artemisinin levels were measured after 7-14 days incubation, and production was significantly reduced by each inhibitor compared to controls, thus, it appears that IPP from both pathways is used in artemisinin production. Also when grown in miconazole, an inhibitor of sterol biosynthesis, there was a significant increase in artemisinin compared to controls suggesting that carbon was shifted from sterols into sesquiterpenes. Collectively these results indicate that artemisinin is probably biosynthesized from IPP pools from both the plastid and the cytosol, and that carbon from competing pathways can be channeled toward sesquiterpenes. This information will help advance our understanding of the regulation of in planta production of artemisinin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.