ArticleMolecular and cellular biology2007
The SCL +40 enhancer targets the midbrain together with primitive and definitive hematopoiesis and is regulated by SCL and GATA proteins.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 44 citations in OpenAlex.
- Article
- SCL/TAL1 cooperates with Polycomb RYBP-PRC1 to suppress alternative lineages in blood-fated cells.Nature communications · 2018Article
- A quantitative proteomics approach identifies ETV6 and IKZF1 as new regulators of an ERG-driven transcriptional network.Nucleic acids research · 2016Article
- Genomic Alterations of Non-Coding Regions Underlie Human Cancer: Lessons from T-ALL.Trends in molecular medicine · 2016Review
- Review
- Transcriptional network control of normal and leukaemic haematopoiesis.Experimental cell research · 2014Review
- Single-cell analyses of regulatory network perturbations using enhancer-targeting TALEs suggest novel roles for PU.1 during haematopoietic specification.Development (Cambridge, England) · 2014Article
- Mechanisms regulating GABAergic neuron development.Cellular and molecular life sciences : CMLS · 2014Review
- Aberrant TAL1 activation is mediated by an interchromosomal interaction in human T-cell acute lymphoblastic leukemia.Leukemia · 2014Article
- Analysis of hundreds of cis-regulatory landscapes at high resolution in a single, high-throughput experiment.Nature genetics · 2014Article
- Mesp1 patterns mesoderm into cardiac, hematopoietic, or skeletal myogenic progenitors in a context-dependent manner.Cell stem cell · 2013Article
- Identification of biologically relevant enhancers in human erythroid cells.The Journal of biological chemistry · 2013Article
- Impaired in vitro erythropoiesis following deletion of the Scl (Tal1) +40 enhancer is largely compensated for in vivo despite a significant reduction in expression.Molecular and cellular biology · 2013Article
- The aromatase gene CYP19A1: several genetic and functional lines of evidence supporting a role in reading, speech and language.Behavior genetics · 2012Article
- Deletion of the Scl +19 enhancer increases the blood stem cell compartment without affecting the formation of mature blood lineages.Experimental hematology · 2012Article
- A core erythroid transcriptional network is repressed by a master regulator of myelo-lymphoid differentiation.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Article
- Genome-wide analysis of simultaneous GATA1/2, RUNX1, FLI1, and SCL binding in megakaryocytes identifies hematopoietic regulators.Developmental cell · 2011Article
- Transcriptional regulation of haematopoietic transcription factors.Stem cell research & therapy · 2011Review
- cis-Regulatory remodeling of the SCL locus during vertebrate evolution.Molecular and cellular biology · 2010Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The SCL/Tal-1 gene encodes a basic helix-loop-helix transcription factor with key roles in hematopoietic and neural development. SCL is expressed in, and required for, both primitive and definitive erythropoiesis. Thus far, we have identified only one erythroid SCL enhancer. Located 40 kb downstream of exon 1a, the +40 enhancer displays activity in primitive erythroblasts. We demonstrate here that a 3.7-kb fragment containing this element also targets expression to the midbrain, a known site of endogenous SCL expression. Although the 3.7-kb construct was active in primitive, but not definitive, erythroblasts, a larger 5.0-kb fragment, encompassing the 3.7-kb region, was active in both fetal and adult definitive hematopoietic cells. This included Ter119+ erythroid cells along with fetal liver erythroid and myeloid progenitors. Unlike two other SCL hematopoietic enhancers (+18/19 and -4), +40 enhancer transgenes were inactive in the endothelium. A conserved 400-bp core region, essential for both hematopoietic and midbrain +40 enhancer activity in embryos, relied on two GATA/E-box motifs and was bound in vivo by GATA-1 and SCL in erythroid cells. These results suggest a model in which the SCL +18/19 and/or -4 enhancers initiate SCL expression in early mesodermal derivatives capable of generating blood and endothelium, with subsequent activation of the +40 enhancer via an autoregulatory loop.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.