ArticleMolecular and cellular biology2007
A conditional role of U2AF in splicing of introns with unconventional polypyrimidine tracts.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Genome-wide modulation of alternative splicing by a predicted alpha helix in U2AF2.Nucleic acids research · 2025Article
- Evolution of the Early Spliceosomal Complex-From Constitutive to Regulated Splicing.International journal of molecular sciences · 2021Review
- Genetic diversity analysis of specialty glutinous and low-amylose rice (Journal of biosciences · 2020Article
- Genomic functions of U2AF in constitutive and regulated splicing.RNA biology · 2015Article
- Structure-guided U2AF65 variant improves recognition and splicing of a defective pre-mRNA.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Mechanisms for U2AF to define 3' splice sites and regulate alternative splicing in the human genome.Nature structural & molecular biology · 2014Article
- Characterization and in vivo functional analysis of the Schizosaccharomyces pombe ICLN gene.Molecular and cellular biology · 2014Article
- The survival motor neuron gene smn-1 interacts with the U2AF large subunit gene uaf-1 to regulate Caenorhabditis elegans lifespan and motor functions.RNA biology · 2014Article
- Splicing functions and global dependency on fission yeast slu7 reveal diversity in spliceosome assembly.Molecular and cellular biology · 2013Article
- Strict 3' splice site sequence requirements for U2 snRNP recruitment after U2AF binding underlie a genetic defect leading to autoimmune disease.RNA (New York, N.Y.) · 2011Article
- Genomic mRNA profiling reveals compensatory mechanisms for the requirement of the essential splicing factor U2AF.Molecular and cellular biology · 2011Article
- Factors affecting splicing strength of yeast genes.Comparative and functional genomics · 2011Article
- Specific splicing defects in S. pombe carrying a degron allele of the Survival of Motor Neuron gene.The EMBO journal · 2010Article
- Differential 3' splice site recognition of SMN1 and SMN2 transcripts by U2AF and U2 snRNP.RNA (New York, N.Y.) · 2009Article
- A novel insertion variant of the human IL-23 receptor-alpha chain transcript.Genes and immunity · 2008Article
- Identification of motifs that function in the splicing of non-canonical introns.Genome biology · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recognition of polypyrimidine (Py) tracts typically present between the branch point and the 3' splice site by the large subunit of the essential splicing factor U2AF is a key early step in pre-mRNA splicing. Diverse intronic sequence arrangements exist, however, including 3' splice sites lacking recognizable Py tracts, which raises the question of how general the requirement for U2AF is for various intron architectures. Our analysis of fission yeast introns in vivo has unexpectedly revealed that whereas introns lacking Py tracts altogether remain dependent on both subunits of U2AF, introns with long Py tracts, unconventionally positioned upstream of branch points, are unaffected by U2AF inactivation. Nevertheless, mutation of these Py tracts causes strong dependence on the large subunit U2AF59. We also find that Py tract diversity influences the requirement for the conserved C-terminal domain of U2AF59 (RNA recognition motif 3), which has been implicated in protein-protein interactions with other splicing factors. Together, these results suggest that in addition to Py tract binding by U2AF, supplementary mechanisms of U2AF recruitment and 3' splice site identification exist to accommodate diverse intron architectures, which have gone unappreciated in biochemical studies of model pre-mRNAs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.