ArticleMolecular and cellular biology2007
Acetylation-induced transcription is required for active DNA demethylation in methylation-silenced genes.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 91 citations in OpenAlex.
- A New Complexity Layer: DNA Methylation and the Predictive Impact of Epigenetic Tests.International journal of molecular sciences · 2026Review
- High levels of histone acetylation modifications promote the formation of PGCs.Poultry science · 2025Article
- Epigenetic Regulatory Processes Involved in the Establishment and Maintenance of Skin Homeostasis-The Role of Microbiota.International journal of molecular sciences · 2025Review
- 5-Aza-2'-Deoxycytidine Regulates White Adipocyte Browning by Modulating miRNA-133a/Prdm16.Metabolites · 2022Article
- Article
- Identification of Tissue-Specific Gene Clusters Induced by DNA Demethylation in Lung Adenocarcinoma: More Than Germline Genes.Cancers · 2022Article
- The Activation of Protamine 1 Using Epigenome Editing Decreases the Proliferation of Tumorigenic Cells.Frontiers in genome editing · 2022Article
- Article
- Local DNA methylation helps to regulate muscle sirtuin 1 gene expression across seasons and advancing age in gilthead sea bream (Frontiers in zoology · 2020Article
- Asymmetric Inheritance of Cell Fate Determinants: Focus on RNA.Non-coding RNA · 2019Review
- Review
- Concerted Flexibility of Chromatin Structure, Methylome, and Histone Modifications along with Plant Stress Responses.Biology · 2017Review
- Stress and the Emerging Roles of Chromatin Remodeling in Signal Integration and Stable Transmission of Reversible Phenotypes.Frontiers in behavioral neuroscience · 2017Review
- Reciprocal Effects on Neurocognitive and Metabolic Phenotypes in Mouse Models of 16p11.2 Deletion and Duplication Syndromes.PLoS genetics · 2016Article
- DNA methylation regulates bromodomain-containing protein 2 expression during adipocyte differentiation.Molecular and cellular biochemistry · 2015Article
- An epigenetic hypothesis for the genomic memory of pain.Frontiers in cellular neuroscience · 2015Article
- Histone deacetylase inhibitor improves the development and acetylation levels of cat-cow interspecies cloned embryos.Cellular reprogramming · 2013Article
- Ing1 functions in DNA demethylation by directing Gadd45a to H3K4me3.Genes & development · 2013Article
- DNA methylation and its basic function.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2013Review
- How do environments talk to genes?Nature neuroscience · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A hallmark of vertebrate genes is that actively transcribed genes are hypomethylated in critical regulatory sequences. However, the mechanisms that link gene transcription and DNA hypomethylation are unclear. Using a trichostatin A (TSA)-induced replication-independent demethylation assay with HEK 293 cells, we show that RNA transcription is required for DNA demethylation. Histone acetylation precedes but is not sufficient to trigger DNA demethylation. Following histone acetylation, RNA polymerase II (RNAP II) interacts with the methylated promoter. Inhibition of RNAP II transcription with actinomycin D, alpha-amanitin, or CDK7-specific small interfering RNA inhibits DNA demethylation. H3 trimethyl lysine 4 methylation, a marker of actively transcribed genes, was associated with the cytomegalovirus promoter only after demethylation. TSA-induced demethylation of the endogenous cancer testis gene GAGE follows a similar sequence of events and is dependent on RNA transcription as well. These data suggest that DNA demethylation follows rather than precedes early transcription and point towards a novel function for DNA demethylation as a memory of actively transcribed genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.