ArticleMolecular immunology2008
Complex regulation of human cathelicidin gene expression: novel splice variants and 5'UTR negative regulatory element.
Article in Molecular immunology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
- Antimicrobial production by perifollicular dermal preadipocytes is essential to the pathophysiology of acne.Science translational medicine · 2022Article
- Reduced vitamin D-induced cathelicidin production and killing ofFrontiers in immunology · 2022Article
- Antimicrobial peptides and the gut microbiome in inflammatory bowel disease.World journal of gastroenterology · 2021Article
- Dietary Nutrients Mediate Intestinal Host Defense Peptide Expression.Advances in nutrition (Bethesda, Md.) · 2020Review
- Cathelicidin Host Defense Peptides and Inflammatory Signaling: Striking a Balance.Frontiers in microbiology · 2020Review
- Retinoids Enhance the Expression of Cathelicidin Antimicrobial Peptide during Reactive Dermal Adipogenesis.Journal of immunology (Baltimore, Md. : 1950) · 2019Article
- Avian antimicrobial host defense peptides: from biology to therapeutic applications.Pharmaceuticals (Basel, Switzerland) · 2014Article
- Regulation of antimicrobial peptide gene expression by nutrients and by-products of microbial metabolism.European journal of nutrition · 2012Review
- Alveolar macrophage cathelicidin deficiency in severe sarcoidosis.Journal of innate immunity · 2012Article
- Coordinate activation of inflammatory gene networks, alveolar destruction and neonatal death in AKNA deficient mice.Cell research · 2011Article
- Bcl-3 acts as an innate immune modulator by controlling antimicrobial responses in keratinocytes.The Journal of investigative dermatology · 2009Article
- cAMP stringently regulates human cathelicidin antimicrobial peptide expression in the mucosal epithelial cells by activating cAMP-response element-binding protein, AP-1, and inducible cAMP early repressor.The Journal of biological chemistry · 2009Article
- The roles of antimicrobial peptides in innate host defense.Current pharmaceutical design · 2009Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Cationic antimicrobial peptides play important roles in host defense, linking innate and adaptive immunity. hCAP18, the only human antimicrobial cathelicidin, consists of a conserved N-terminal cathelin-like domain and a C-terminal peptide, LL-37. Expression is regulated during myeloid differentiation, and tightly controlled during infection and inflammation, suggesting active regulation. Using 5' RACE (rapid amplification of cDNA ends), multiple transcription initiation sites were identified, as well as new splice variants leading to novel augmentations of hCAP18 amino acid composition in bone marrow but not peripheral blood neutrophils. Having expressed hCAP18 promoter constructs in cell lines, we found that full-length (-1739) and truncated (-978) promoter constructs had lower luciferase activities than 5'UTR deletion constructs. Transient transfection of progressively deleted constructs in the non-permissive K562 cell line led us to identify a negative regulatory element within the 53 bp immediately upstream of the ATG of hCAP18. Additionally, transient transfection of 5' deletion constructs identified a positive regulatory element within the 101 bases 5' of promoter sequence containing two GT-boxes. Negative and positive regulatory elements within the hCAP18 gene promoter provide new insights into the possible molecular basis of myeloid gene expression.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.