ArticleThe Biochemical journal2007
Determination of protein regions responsible for interactions of amelogenin with CD63 and LAMP1.
Article in The Biochemical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 44 citations in OpenAlex.
- PKCα regulates the secretion of PDL1-carrying small extracellular vesicles in a p53-dependent manner.Cell death & disease · 2025Article
- Ameloblastin and its multifunctionality in amelogenesis: A review.Matrix biology : journal of the International Society for Matrix Biology · 2024Review
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- Amelogenin Downregulates Interferon Gamma-Induced Major Histocompatibility Complex Class II Expression Through Suppression of Euchromatin Formation in the Class II Transactivator Promoter IV Region in Macrophages.Frontiers in immunology · 2020Article
- Ameloblastin Binds to Phospholipid Bilayers via a Helix-Forming Motif within the Sequence Encoded by Exon 5.ACS omega · 2019Article
- DENTAL ENAMEL FORMATION AND IMPLICATIONS FOR ORAL HEALTH AND DISEASE.Physiological reviews · 2017Review
- MiR-153 Regulates Amelogenesis by Targeting Endocytotic and Endosomal/lysosomal Pathways-Novel Insight into the Origins of Enamel Pathologies.Scientific reports · 2017Article
- Enamel ribbons, surface nodules, and octacalcium phosphate in C57BL/6Molecular genetics & genomic medicine · 2016Article
- Article
- Genome-wide analysis of miRNA and mRNA transcriptomes during amelogenesis.BMC genomics · 2014Article
- Adaptor protein complex 2-mediated, clathrin-dependent endocytosis, and related gene activities, are a prominent feature during maturation stage amelogenesis.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2013Article
- Amelogenins: Multi-Functional Enamel Matrix Proteins and Their Binding Partners.Journal of oral biosciences · 2011Article
- Structural analysis of a repetitive protein sequence motif in strepsirrhine primate amelogenin.PloS one · 2011Article
- Ameloblastin regulates osteogenic differentiation by inhibiting Src kinase via cross talk between integrin beta1 and CD63.Molecular and cellular biology · 2011Article
- Full length amelogenin binds to cell surface LAMP-1 on tooth root/periodontium associated cells.Archives of oral biology · 2010Article
- Mutations in the beta propeller WDR72 cause autosomal-recessive hypomaturation amelogenesis imperfecta.American journal of human genetics · 2009Article
- Regeneration of bone and periodontal ligament induced by recombinant amelogenin after periodontitis.Journal of cellular and molecular medicine · 2009Article
- The cell adhesion molecule nectin-1 is critical for normal enamel formation in mice.Human molecular genetics · 2008Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
The enamel matrix protein amelogenin is secreted by ameloblasts into the extracellular space to guide the formation of highly ordered hydroxyapatite mineral crystallites, and, subsequently, is almost completely removed during mineral maturation. Amelogenin interacts with the transmembrane proteins CD63 and LAMP (lysosome-associated membrane protein) 1, which are involved in endocytosis. Exogenously added amelogenin has been observed to move rapidly into CD63/LAMP1-positive vesicles in cultured cells. In the present study, we demonstrate the protein region defined by amino acid residues 103-205 for CD63 interacts not only with amelogenin, but also with other enamel matrix proteins (ameloblastin and enamelin). A detailed characterization of binding regions in amelogenin, CD63 and LAMP1 reveals that the amelogenin region defined by residues PLSPILPELPLEAW is responsible for the interaction with CD63 through residues 165-205, with LAMP1 through residues 226-251, and with the related LAMP2 protein through residues 227-259. We predict that the amelogenin binding region is: (i) hydrophobic; (ii) largely disordered; and (iii) accessible to the external environment. In contrast, the binding region of CD63 is likely to be organized in a '7' shape within the mushroom-like structure of CD63 EC2 (extracellular domain 2). In vivo, the protein interactions between the secreted enamel matrix proteins with the membrane-bound proteins are likely to occur at the specialized secretory surfaces of ameloblast cells called Tomes' processes. Such protein-protein interactions may be required to establish short-term order of the forming matrix and/or to mediate feedback signals to the transcriptional machinery of ameloblasts and/or to remove matrix protein debris during enamel biomineralization.
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