ArticleGenome biology2007
Genomic mapping of Suppressor of Hairy-wing binding sites in Drosophila.
Article in Genome biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 59 citations in OpenAlex.
- Article
- Phosphorylated histone variant γH2Av is associated with chromatin insulators in Drosophila.PLoS genetics · 2022Article
- Mutational analysis identifies functional Rap1, Su(Hw), and CTCF insulator sites in Arabidopsis thaliana.Plant cell reports · 2020Article
- Dissecting the binding mechanisms of transcription factors to DNA using a statistical thermodynamics framework.Computational and structural biotechnology journal · 2020Article
- The Role of Insulators in Transgene Transvection inGenetics · 2019Article
- TAD-free analysis of architectural proteins and insulators.Nucleic acids research · 2018Article
- An Organizational Hub of Developmentally Regulated Chromatin Loops in the Drosophila Antennapedia Complex.Molecular and cellular biology · 2015Article
- Two new insulator proteins, Pita and ZIPIC, target CP190 to chromatin.Genome research · 2015Article
- Surviving an identity crisis: a revised view of chromatin insulators in the genomics era.Biochimica et biophysica acta · 2014Review
- Copy number variations of the extensively amplified Y-linked genes, HSFY and ZNF280BY, in cattle and their association with male reproductive traits in Holstein bulls.BMC genomics · 2014Article
- The insulator protein Suppressor of Hairy-wing is an essential transcriptional repressor in the Drosophila ovary.Development (Cambridge, England) · 2013Article
- Exogenous gypsy insulator sequences modulate transgene expression in the malaria vector mosquito, Anopheles stephensi.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The influence of transcription factor competition on the relationship between occupancy and affinity.PloS one · 2013Article
- Effective blocking of the white enhancer requires cooperation between two main mechanisms suggested for the insulator function.PLoS genetics · 2013Article
- Nature and function of insulator protein binding sites in the Drosophila genome.Genome research · 2012Article
- Genome-wide studies of the multi-zinc finger Drosophila Suppressor of Hairy-wing protein in the ovary.Nucleic acids research · 2012Article
- The tumour suppressor L(3)mbt inhibits neuroepithelial proliferation and acts on insulator elements.Nature cell biology · 2011Article
- The role of the Suppressor of Hairy-wing insulator protein in Drosophila oogenesis.Developmental biology · 2011Article
- Genome-wide analysis of the binding of the Hox protein Ultrabithorax and the Hox cofactor Homothorax in Drosophila.PloS one · 2011Article
- Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
backgroundInsulator elements are proposed to play a key role in the organization of the regulatory architecture of the genome. In Drosophila, one of the best studied is the gypsy retrotransposon insulator, which is bound by the Suppressor of Hairy-wing (Su [Hw]) transcriptional regulator. Immunolocalization studies suggest that there are several hundred Su(Hw) sites in the genome, but few of these endogenous Su(Hw) binding sites have been identified.
resultsWe used chromatin immunopurification with genomic microarray analysis to identify in vivo Su(Hw) binding sites across the 3 megabase Adh region. We find 60 sites, and these enabled the construction of a robust new Su(Hw) binding site consensus. In contrast to the gypsy insulator, which contains tightly clustered Su(Hw) binding sites, endogenous sites generally occur as isolated sites. These endogenous sites have three key features. In contrast to most analyses of DNA-binding protein specificity, we find that strong matches to the binding consensus are good predictors of binding site occupancy. Examination of occupancy in different tissues and developmental stages reveals that most Su(Hw) sites, if not all, are constitutively occupied, and these isolated Su(Hw) sites are generally highly conserved. Analysis of transcript levels in su(Hw) mutants indicate widespread and general changes in gene expression. Importantly, the vast majority of genes with altered expression are not associated with clustering of Su(Hw) binding sites, emphasizing the functional relevance of isolated sites.
conclusionTaken together, our in vivo binding and gene expression data support a role for the Su(Hw) protein in maintaining a constant genomic architecture.
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