ArticleMalaria journal2007
Comparison of PCR-based detection of Plasmodium falciparum infections based on single and multicopy genes.
Article in Malaria journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Overreliance on Plasmodium 18S rRNA gene for malaria molecular diagnosis-inferences from systematic review.Malaria journal · 2025Pooled it
- Prospective evaluation of artemether-lumefantrine for the treatment of non-falciparum and mixed-species malaria in Gabon.Malaria journal · 2012Trial
- Mechanistic insights into subclinicaliScience · 2026Review
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- Detection ofDiagnostics (Basel, Switzerland) · 2023Article
- A Novel Nested Multiplex Polymerase Chain Reaction Assay for Malaria Diagnosis Using the Hydroxymethyl Dihydropterin Pyrophosphokinase-Dihydropteroate Synthase (hppk-dhps) Gene.The American journal of tropical medicine and hygiene · 2023Article
- Accuracy of diagnosis among clinical malaria patients: comparing microscopy, RDT and a highly sensitive quantitative PCR looking at the implications for submicroscopic infections.Malaria journal · 2023Article
- DNA recovery from used malaria RDT to detect Plasmodium species and to assess Plasmodium falciparum genetic diversity: a pilot study in Madagascar.Malaria journal · 2022Article
- Molecular Identification and Characterization of Plasmodium ovale curtisi in Field Isolates from Symptomatic Children in North-Central Nigeria.Acta parasitologica · 2021Article
- Template copy number and the sensitivity of quantitative PCR for Plasmodium falciparum in asymptomatic individuals.Malaria journal · 2020Article
- Droplet digital polymerase chain reaction (ddPCR) for the detection of Plasmodium knowlesi and Plasmodium vivax.Malaria journal · 2020Article
- Cell lysis via acoustically oscillating sharp edges.Lab on a chip · 2019Article
- Genetic Diversity of CD14 Promoter Gene Polymorphism (Infectious diseases · 2017Article
- Human malaria diagnosis using a single-step direct-PCR based on the Plasmodium cytochrome oxidase III gene.Malaria journal · 2016Article
- Ultra-sensitive detection of Plasmodium falciparum by amplification of multi-copy subtelomeric targets.PLoS medicine · 2015Article
- A multi-detection assay for malaria transmitting mosquitoes.Journal of visualized experiments : JoVE · 2015Article
- Comparison of three PCR-based assays for the non-invasive diagnosis of malaria: detection of Plasmodium parasites in blood and saliva.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2014Article
- Prevalence of Plasmodium falciparum Parasitaemia and Its Correlation with Haematological Parameters among HIV-Positive Individuals in Nigeria.Journal of tropical medicine · 2014Article
- Development and evaluation of a 28S rRNA gene-based nested PCR assay for P. falciparum and P. vivax.Pathogens and global health · 2013Article
- Assessment of Clinical Diagnosis, Microscopy, Rapid Diagnostic Tests, and Polymerase Chain Reaction in the Diagnosis of Plasmodium falciparum in Nigeria.Malaria research and treatment · 2013Article
Corrections and comments
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6 authors.
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Abstract
PCR-based assays are the most sensitive and specific methods to detect malaria parasites. This study compared the diagnostic accuracy of three PCR-based assays that do not only differ in their sequence target, but also in the number of copies of their target region, for the detection of Plasmodium falciparum in 401 individuals living in a malaria-endemic area in Nigeria. Compared to a composite reference generated from results of all the 3 PCR assays, the stevor gene amplification had a sensitivity of 100% (Kappa = 1; 95% CI = 1.000-1.000), 83% (Kappa = 0.718; 95% CI = 0.648-0.788) by SSUrRNA gene PCR and 71% (Kappa = 0.552; 95% CI = 0.478-0.627) by the msa-2 gene amplification. Results from this study indicate that the stevor gene amplification is the most sensitive technique for the detection of P. falciparum. This assay may be an important reference standard, especially when a confirmatory technique with high sensitivity and specificity is needed for ruling out P. falciparum infection.
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