ArticleThe Biochemical journal2007
A stress-induced rice (Oryza sativa L.) beta-glucosidase represents a new subfamily of glycosyl hydrolase family 5 containing a fascin-like domain.
Article in The Biochemical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 52 citations in OpenAlex.
- Genome-Wide Identification of the Soybean GH5 Gene Family and Functional Analysis ofPlants (Basel, Switzerland) · 2026Article
- Phylogenetic and Functional Analyses of WheatBiology · 2026Article
- Characterization of a Rice GH5_11 Gene Associated with Endosperm and Seed Traits.Plants (Basel, Switzerland) · 2025Article
- Isolation and identification of core-genes in barley roots under low phosphorus stress.Molecular biology reports · 2025Article
- Non-Catalytic Domains of Glycoside Hydrolase Family 5 fromJournal of microbiology and biotechnology · 2025Article
- Integrative physiological, biochemical, and proteomic analysis of the leaves of two cotton genotypes under heat stress.PloS one · 2025Article
- Genome-wide identification and gene expression pattern analysis of the glycoside hydrolase family 1 in Fagopyrum tataricum.BMC plant biology · 2024Article
- Transcriptome Profiling Reveals Molecular Responses to Salt Stress in Common Vetch (Plants (Basel, Switzerland) · 2024Article
- HvBGlu3, a GH1 β-glucosidase enzyme gene, negatively influences β-glucan content in barley grains.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
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- Computational genomics insights into cold acclimation in wheat.Frontiers in genetics · 2022Article
- Full-length transcriptome profiling reveals insight into the cold response of two kiwifruit genotypes (A. arguta) with contrasting freezing tolerances.BMC plant biology · 2021Article
- Structure and dynamics analysis of multi-domain putative β-1,4-glucosidase of family 3 glycoside hydrolase (PsGH3) from Pseudopedobacter saltans.Journal of molecular modeling · 2021Article
- Integrated Analysis of Metabolome and Transcriptome Reveals Insights for Cold Tolerance in Rapeseed (Frontiers in plant science · 2021Article
- LSSR1 facilitates seed setting rate by promoting fertilization in rice.Rice (New York, N.Y.) · 2019Article
- Association Analysis of Salt Tolerance in Asiatic cotton (International journal of molecular sciences · 2019Article
- Identification of charcoal rot resistance QTLs in sorghum using association and in silico analyses.Journal of applied genetics · 2018Article
- The structural and functional contributions of β-glucosidase-producing microbial communities to cellulose degradation in composting.Biotechnology for biofuels · 2018Article
- Functional Analysis of a Novel β-(1,3)-Glucanase from Corallococcus sp. Strain EGB Containing a Fascin-Like Module.Applied and environmental microbiology · 2017Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
GH5BG, the cDNA for a stress-induced GH5 (glycosyl hydrolase family 5) beta-glucosidase, was cloned from rice (Oryza sativa L.) seedlings. The GH5BG cDNA encodes a 510-amino-acid precursor protein that comprises 19 amino acids of prepeptide and 491 amino acids of mature protein. The protein was predicted to be extracellular. The mature protein is a member of a plant-specific subgroup of the GH5 exoglucanase subfamily that contains two major domains, a beta-1,3-exoglucanase-like domain and a fascin-like domain that is not commonly found in plant enzymes. The GH5BG mRNA is highly expressed in the shoot during germination and in leaf sheaths of mature plants. The GH5BG was up-regulated in response to salt stress, submergence stress, methyl jasmonate and abscisic acid in rice seedlings. A GUS (glucuronidase) reporter tagged at the C-terminus of GH5BG was found to be secreted to the apoplast when expressed in onion (Allium cepa) cells. A thioredoxin fusion protein produced from the GH5BG cDNA in Escherichia coli hydrolysed various pNP (p-nitrophenyl) glycosides, including beta-D-glucoside, alpha-L-arabinoside, beta-D-fucoside, beta-D-galactoside, beta-D-xyloside and beta-D-cellobioside, as well as beta-(1,4)-linked glucose oligosaccharides and beta-(1,3)-linked disaccharide (laminaribiose). The catalytic efficiency (kcat/K(m)) for hydrolysis of beta-(1,4)-linked oligosaccharides by the enzyme remained constant as the DP (degree of polymerization) increased from 3 to 5. This substrate specificity is significantly different from fungal GH5 exoglucanases, such as the exo-beta-(1,3)-glucanase of the yeast Candida albicans, which may correlate with a marked reduction in a loop that makes up the active-site wall in the Candida enzyme.
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