ArticleBiochemistry2007
Cytotoxic ribonucleases: the dichotomy of Coulombic forces.
Article in Biochemistry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 49 citations in OpenAlex.
- Reductive Methylation: An Alternative to Lysine → Arginine Mutagenesis.Journal of peptide science : an official publication of the European Peptide Society · 2026Article
- Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.International journal of molecular sciences · 2022Article
- Antimicrobial Synergy of a Ribonuclease and a Peptide Secreted by Human Cells.ACS infectious diseases · 2020Article
- Esterification Delivers a Functional Enzyme into a Human Cell.ACS chemical biology · 2019Article
- Biological Activities of Secretory RNases: Focus on Their Oligomerization to Design Antitumor Drugs.Frontiers in immunology · 2019Review
- Updates in the Development of ImmunoRNases for the Selective Killing of Tumor Cells.Biomedicines · 2018Review
- Fine-Tuning Strain and Electronic Activation of Strain-Promoted 1,3-Dipolar Cycloadditions with Endocyclic Sulfamates in SNO-OCTs.Journal of the American Chemical Society · 2017Article
- Boronic Acid for the Traceless Delivery of Proteins into Cells.ACS chemical biology · 2016Article
- Human Cancer Antigen Globo H Is a Cell-Surface Ligand for Human Ribonuclease 1.ACS central science · 2015Article
- Contribution of electrostatics to the binding of pancreatic-type ribonucleases to membranes.Biochemistry · 2013Article
- Human ribonuclease with a pendant poly(ethylene glycol) inhibits tumor growth in mice.Translational oncology · 2013Article
- Fluorogenic probe for constitutive cellular endocytosis.Chemistry & biology · 2013Article
- A novel fully human antitumor immunoRNase resistant to the RNase inhibitor.Protein engineering, design & selection : PEDS · 2013Article
- Interaction of nucleic acids with the glycocalyx.Journal of the American Chemical Society · 2012Article
- Rational design and evaluation of mammalian ribonuclease cytotoxins.Methods in enzymology · 2012Article
- Arginine residues are more effective than lysine residues in eliciting the cellular uptake of onconase.Biochemistry · 2011Article
- Article
- Site-specific PEGylation endows a mammalian ribonuclease with antitumor activity.Cancer biology & therapy · 2011Article
- Potentiation of ribonuclease cytotoxicity by a poly(amidoamine) dendrimer.Bioorganic & medicinal chemistry letters · 2011Article
- Cellular uptake of ribonuclease A relies on anionic glycans.Biochemistry · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Cells tightly regulate their contents. Still, nonspecific Coulombic interactions between cationic molecules and anionic membrane components can lead to adventitious endocytosis. Here, we characterize this process in a natural system. To do so, we create variants of human pancreatic ribonuclease (RNase 1) that differ in net molecular charge. By conjugating a small-molecule latent fluorophore to these variants and using flow cytometry, we are able to determine the kinetic mechanism for RNase 1 internalization into live human cells. We find that internalization increases with solution concentration and is not saturable. Internalization also increases with time to a steady-state level, which varies linearly with molecular charge. In contrast, the rate constant for internalization (t1/2 = 2 h) is independent of charge. We conclude that internalization involves an extracellular equilibrium complex between the cationic proteins and abundant anionic cell-surface molecules, followed by rate-limiting internalization. The enhanced internalization of more cationic variants of RNase 1 is, however, countered by their increased affinity for the cytosolic ribonuclease inhibitor protein, which is anionic. Thus, Coulombic forces mediate extracellular and intracellular equilibria in a dichotomous manner that both endangers cells and defends them from the potentially lethal enzymatic activity of ribonucleases.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.