ArticlePflugers Archiv : European journal of physiology2008
The use of CalciumOrange-5N as a specific marker of mitochondrial Ca2+ in mouse skeletal muscle fibers.
Article in Pflugers Archiv : European journal of physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 24 citations in OpenAlex.
- Review
- Diaphragm Fatigue in SMNΔ7 Mice and Its Molecular Determinants: An Underestimated Issue.International journal of molecular sciences · 2023Article
- Excitation-contraction coupling in mammalian skeletal muscle: Blending old and last-decade research.Frontiers in physiology · 2022Review
- Ageing Causes Ultrastructural Modification to Calcium Release Units and Mitochondria in Cardiomyocytes.International journal of molecular sciences · 2021Article
- Improper Remodeling of Organelles Deputed to CaInternational journal of molecular sciences · 2021Review
- Calcium entry units (CEUs): perspectives in skeletal muscle function and disease.Journal of muscle research and cell motility · 2021Article
- Muscle activity prevents the uncoupling of mitochondria from CaArchives of biochemistry and biophysics · 2019Article
- Age-dependent uncoupling of mitochondria from Ca2⁺ release units in skeletal muscle.Oncotarget · 2015Article
- Tetanic Ca2+ transient differences between slow- and fast-twitch mouse skeletal muscle fibres: a comprehensive experimental approach.Journal of muscle research and cell motility · 2014Article
- The excitation-contraction coupling mechanism in skeletal muscle.Biophysical reviews · 2014Review
- Quantifying SOCE fluorescence measurements in mammalian muscle fibres. The effects of ryanodine and osmotic shocks.Journal of muscle research and cell motility · 2013Article
- Mitochondrial Ca2+-handling in fast skeletal muscle fibers from wild type and calsequestrin-null mice.PloS one · 2013Article
- Lessons from calsequestrin-1 ablation in vivo: much more than a Ca(2+) buffer after all.Journal of muscle research and cell motility · 2011Article
- Differential impact of mitochondrial positioning on mitochondrial Ca(2+) uptake and Ca(2+) spark suppression in skeletal muscle.American journal of physiology. Cell physiology · 2011Article
- Altered myoplasmic Ca(2+) handling in rat fast-twitch skeletal muscle fibres during disuse atrophy.Pflugers Archiv : European journal of physiology · 2010Article
- Factors affecting SOCE activation in mammalian skeletal muscle fibers.The journal of physiological sciences : JPS · 2009Article
- Mitochondria are linked to calcium stores in striated muscle by developmentally regulated tethering structures.Molecular biology of the cell · 2009Article
- Different fibre populations distinguished by their calcium transient characteristics in enzymatically dissociated murine flexor digitorum brevis and soleus muscles.Journal of muscle research and cell motility · 2009Article
- Effect of mitochondria poisoning by FCCP on Ca2+ signaling in mouse skeletal muscle fibers.Pflugers Archiv : European journal of physiology · 2008Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report the use of the fluorescent dye CalciumOrange-5N (CaOr-5N) as a specific mitochondria Ca(2+) marker in enzymatically dissociated mouse FBD muscle fibers. Using laser scanning confocal microscopy and the dyes Mitotracker Green (MTG), di-8-ANEPPS and endoplasmic reticulum tracker green (ERTG), we determined the relative position of mitochondria, transverse tubules and sarcoplasmic reticulum in the sarcomere. Comparison with electron micrographies showed that mitochondria are mostly present at both sides of Z lines and near the triads located at the A-I band border. CaOr-5N fluorescence was mainly distributed in mitochondria, highly co-localised with MTG and basically excluded from the A band space. ERTG localised mostly between the two t-tubules present in each sarcomere. We studied the effect of the protonophore FCCP using CaOr-5N to measure mitochondrial Ca(2+) and JC-1 dye to measure mitochondria inner membrane potential (DeltaPsi(m)). After FCCP treatment, the CaOr-5N fluorescence diminished by about 33% in 80 s, while JC-1 fluorescence diminished by 36% in 200 s. Our results show the loss of Ca(2+) from mitochondria when DeltaPsi(m) is depolarised and demonstrate the usefulness of CaOr-5N to mark mitochondrial [Ca(2+)](m).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.