ArticleDiabetologia2007
An adenylate kinase is involved in KATP channel regulation of mouse pancreatic beta cells.
Article in Diabetologia, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 32 citations in OpenAlex.
- The role of the adenylate kinase 5 gene in various diseases and cancer.Journal of clinical and translational science · 2024Review
- Kinetic and data-driven modeling of pancreatic β-cell central carbon metabolism and insulin secretion.PLoS computational biology · 2022Article
- Yield, cell composition, and function of islets isolated from different ages of neonatal pigs.Frontiers in endocrinology · 2022Article
- Improving the Function and Engraftment of Transplanted Pancreatic Islets Using Pulsed Focused Ultrasound Therapy.Scientific reports · 2019Article
- Evidence against a Ca(2+)-induced potentiation of dehydrogenase activity in pancreatic beta-cells.Pflugers Archiv : European journal of physiology · 2015Article
- Oscillations of sub-membrane ATP in glucose-stimulated beta cells depend on negative feedback from Ca(2+).Diabetologia · 2013Article
- AMPK modulates glucose-sensing in insulin-secreting cells by altered phosphotransfer to KATP channels.Journal of bioenergetics and biomembranes · 2013Article
- Rapid functional evaluation of beta-cells by extracellular recording of membrane potential oscillations with microelectrode arrays.Pflugers Archiv : European journal of physiology · 2011Article
- Oxidative stress and beta-cell dysfunction.Pflugers Archiv : European journal of physiology · 2010Review
- K(ATP) channelopathies in the pancreas.Pflugers Archiv : European journal of physiology · 2010Review
- Adenylate kinase and AMP signaling networks: metabolic monitoring, signal communication and body energy sensing.International journal of molecular sciences · 2009Review
- Activation of the Na+/K+-ATPase by insulin and glucose as a putative negative feedback mechanism in pancreatic beta-cells.Pflugers Archiv : European journal of physiology · 2009Article
- Article
- Neonatal diabetes mellitus.Endocrine reviews · 2008Review
- Cytosolic adenylate kinases regulate K-ATP channel activity in human beta-cells.Biochemical and biophysical research communications · 2008Article
- Metabolic syndrome induces changes in KATP-channels and calcium currents in pancreatic β-cells.IsletsArticle
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aims/hypothesisIn a previous study, we demonstrated that a creatine kinase (CK) modulates K(ATP) channel activity in pancreatic beta cells. To explore phosphotransfer signalling pathways in more detail, we examined whether K(ATP) channel regulation in beta cells is determined by a metabolic interaction between adenylate kinase (AK) and CK.
methodsSingle channel activity was measured with the patch-clamp technique in the inside-out (i/o) and open-cell attached (oca) configuration.
resultsThe ATP sensitivity of K(ATP) channels was higher in i/o patches than in permeabilised beta cells (oca). One reason for this observation could be that the local ATP:ADP ratio in the proximity of the channels is determined by factors not active in i/o patches. AMP (0.1 mmol/l) clearly increased open channel probability in the presence of ATP (0.125 mmol/l) in permeabilised cells but not in excised patches. This suggests that AK-catalysed ADP production in the vicinity of the channels is involved in K(ATP) channel regulation. The observation that the stimulatory effect of AMP on K(ATP) channels was prevented by the AK inhibitor P (1),P (5)-di(adenosine-5')pentaphosphate (Ap(5)A; 20 micromol/l) and abolished in the presence of the non-metabolisable ATP analogue adenosine 5'-(beta,gamma-imido)triphosphate tetralithium salt (AMP-PNP; 0.12 mmol/l) strengthens this idea. In beta cells from AK1 knockout mice, the effect of AMP was less pronounced, though not completely suppressed. The increase in K(ATP) channel activity induced by AMP in the presence of ATP was outweighed by phosphocreatine (1 mmol/l). We suggest that this is due to an elevation of the ATP concentration by CK. CONCLUSIONS/
interpretationWe propose that phosphotransfer events mediated by AK and CK play an important role in determining the effective concentrations of ATP and ADP in the microenvironment of pancreatic beta cell K(ATP) channels. Thus, these enzymes determine the open probability of K(ATP) channels and eventually the actual rate of insulin secretion.
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