ArticleJournal of bacteriology2007
Changes in nucleoid morphology and origin localization upon inhibition or alteration of the actin homolog, MreB, of Vibrio cholerae.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 43 citations in OpenAlex.
- Ploidy inGenes · 2023Review
- CrvA and CrvB form a curvature-inducing module sufficient to induce cell-shape complexity in Gram-negative bacteria.Nature microbiology · 2021Article
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- Targeting the Bacterial Cytoskeleton of theInternational journal of molecular sciences · 2018Article
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- YodL and YisK Possess Shape-Modifying Activities That Are Suppressed by Mutations in Bacillus subtilis mreB and mbl.Journal of bacteriology · 2016Article
- Route of intrabacterial nanotransportation system for CagA in Helicobacter pylori.Medical molecular morphology · 2015Article
- Multidimensional view of the bacterial cytoskeleton.Journal of bacteriology · 2013Review
- The helical MreB cytoskeleton in Escherichia coli MC1000/pLE7 is an artifact of the N-Terminal yellow fluorescent protein tag.Journal of bacteriology · 2012Article
- Electron tomography of cells.Quarterly reviews of biophysics · 2012Review
- Article
- Mechanical control of bacterial cell shape.Biophysical journal · 2011Article
- High-throughput, subpixel precision analysis of bacterial morphogenesis and intracellular spatio-temporal dynamics.Molecular microbiology · 2011Article
- Long helical filaments are not seen encircling cells in electron cryotomograms of rod-shaped bacteria.Biochemical and biophysical research communications · 2011Article
- Participation of chromosome segregation protein ParAI of Vibrio cholerae in chromosome replication.Journal of bacteriology · 2011Article
- Spiral architecture of the nucleoid in Bdellovibrio bacteriovorus.Journal of bacteriology · 2011Article
- The structure and function of bacterial actin homologs.Cold Spring Harbor perspectives in biology · 2010Review
- MreB drives de novo rod morphogenesis in Caulobacter crescentus via remodeling of the cell wall.Journal of bacteriology · 2010Article
- A22 disrupts the bacterial actin cytoskeleton by directly binding and inducing a low-affinity state in MreB.Biochemistry · 2009Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
MreB is an actin homolog required for the morphogenesis of most rod-shaped bacteria and for other functions, including chromosome segregation. In Caulobacter crescentus and Escherichia coli, the protein seems to play a role in the segregation of sister origins, but its role in Bacillus subtilis chromosome segregation is less clear. To help clarify its role in segregation, we have here studied the protein in Vibrio cholerae, whose chromosome I segregates like the one in C. crescentus and whose chromosome II like the one in E. coli or B. subtilis. The properties of Vibrio MreB were similar to those of its homologs in other bacteria in that it formed dynamic helical filaments, was essential for viability, and was inhibited by the drug A22. Wild-type (WT) cells exposed to A22 became spherical and larger. The nucleoids enlarged correspondingly, and the origin positions for both the chromosomes no longer followed any fixed pattern. However, the sister origins separated, unlike the situation in other bacteria. In mutants isolated as A22 resistant, the nucleoids in some cases appeared compacted even when the cell shape was nearly normal. In these cells, the origins of chromosome I were at the distal edges of the nucleoid but not all the way to the poles where they normally reside. The sister origins of chromosome II also separated less. Thus, it appears that the inhibition or alteration of Vibrio MreB can affect both the nucleoid morphology and origin localization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.