ArticleBiophysical journal2007
Simultaneous tether extraction from endothelial cells and leukocytes: observation, mechanics, and significance.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- Combined TIRF and 3D Super-Resolution Microscopy for Nanoscopic Characterization of Adhesion Molecules on Microvilli.Analytical chemistry · 2026Article
- Biomechanics of Neutrophil Tethers.Life (Basel, Switzerland) · 2021Review
- Stiffness based enrichment of leukemia cells using microfluidics.APL bioengineering · 2020Article
- Endothelial Surface Protrusion by a Point Force.Biophysical journal · 2016Article
- A Review of Cell Adhesion Studies for Biomedical and Biological Applications.International journal of molecular sciences · 2015Review
- Cytoskeleton modification and cholesterol depletion affect membrane properties and caveolae positioning of CHO cells.The Journal of membrane biology · 2014Article
- Neutrophil rolling at high shear: flattening, catch bond behavior, tethers and slings.Molecular immunology · 2013Review
- Tangential tether extraction and spontaneous tether retraction of human neutrophils.Biophysical journal · 2012Article
- Rupture of multiple receptor-ligand bonds: bimodal distribution of bond rupture force.The European physical journal. E, Soft matter · 2012Article
- How cholesterol regulates endothelial biomechanics.Frontiers in physiology · 2012Article
- The constitutive equation for membrane tether extraction.Annals of biomedical engineering · 2010Article
- Temperature modulation of integrin-mediated cell adhesion.Biophysical journal · 2010Article
- Biophysical mechanisms of single-cell interactions with microtopographical cues.Biomedical microdevices · 2010Article
- Rolling cell adhesion.Annual review of cell and developmental biology · 2010Review
- Combined atomic force microscopy and side-view optical imaging for mechanical studies of cells.Nature methods · 2009Article
- Validation, In-Depth Analysis, and Modification of the Micropipette Aspiration Technique.Cellular and molecular bioengineering · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
It has been hypothesized, from earlier studies on single-tether extraction from individual leukocytes and human umbilical vein endothelial cells, that during rolling of leukocytes on the endothelium, simultaneous extraction of membrane nanotubes (tethers) occurs, resulting in enhancement of the force decrease on the adhesive bond. In this study, using the micropipette aspiration technique and fluorescence microscopy, we show that tethers are indeed extracted simultaneously when an endothelial cell and a leukocyte are separated after brief contact and adhesion, and the endothelial cell contributes much more to the composite tether length. In addition, the constitutive relationship for simultaneous tether extraction is determined with neutrophils and T-lymphocytes as force transducers, and cytokine-stimulated human umbilical vein and dermal microvascular endothelial cells as substrates, respectively. This relationship is consistent with that derived theoretically from the constitutive equations for single-tether extraction from either cell alone. Moreover, we show that simultaneous tether extraction was likely terminated by receptor-ligand bond dissociation. With a biomechanical model of leukocyte rolling, we predict the force history of the adhesive receptor-ligand bond and show that it is remarkably similar for different leukocyte-endothelial cell pairs. Simultaneous tether extraction therefore represents a generic mechanism for stabilizing leukocyte rolling on the endothelium.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.