ArticleProceedings of the National Academy of Sciences of the United States of America2007
One at a time, live tracking of NGF axonal transport using quantum dots.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 166 papers.
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Who cites it
166 citing papers in PubMed.
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- Direct microsecond wide-field single-molecule tracking and super-resolution mapping via CCD vertical shift.Nature communications · 2025Article
- Visualising sub-second dynamics of nanoparticle extravasationbioRxiv : the preprint server for biology · 2025Article
- Nanometer-resolution tracking of single cargo reveals dynein motor mechanisms.Nature chemical biology · 2025Article
- Neural Tracing Protein-Functionalized Nanoparticles Capable of Fast Retrograde Axonal Transport in Live Neurons.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- Real-Time Tracking of Vesicles in Living Cells Reveals That Tau-Hyperphosphorylation Suppresses Unidirectional Transport by Motor Proteins.Chemical & biomedical imaging · 2024Article
- Interplay Between Intracellular Transport Dynamics and Liquid‒Liquid Phase Separation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Multicolor Long-Term Single-Particle Tracking Using 10 nm Upconverting Nanoparticles.Nano letters · 2024Article
- Neurotrophins in carotid atherosclerosis and stenting.Annals of medicine · 2023Article
- Multidimensional Super-Resolution Microscopy Unveils Nanoscale Surface Aggregates in the Aging of FUS Condensates.Journal of the American Chemical Society · 2023Article
- Optogenetic dissection of RET signaling reveals robust activation of ERK and enhanced filopodia-like protrusions of regenerating axons.Molecular brain · 2023Article
- Failure to Thrive: Impaired BDNF Transport along the Cortical-Striatal Axis in Mouse Q140 Neurons of Huntington's Disease.Biology · 2023Article
- Choosing the Probe for Single-Molecule Fluorescence Microscopy.International journal of molecular sciences · 2022Review
- Molecular Tension Probes to Quantify Cell-Generated Mechanical Forces.Molecules and cells · 2022Review
- Calcineurin and huntingtin form a calcium-sensing machinery that directs neurotrophic signals to the nucleus.Science advances · 2022Article
- Mouse Nerve Growth Factor Injection and Progression Rate in Patients With Amyotrophic Lateral Sclerosis: An Observational Study.Frontiers in neurology · 2022Article
- Microfluidic platforms for single neuron analysis.Materials today. Bio · 2022Review
- Molecular-Scale Dynamics of Long Range Retrograde Brain-Derived Neurotrophic Factor Transport Shaped by Cellular Spatial Context.Frontiers in neuroscience · 2022Article
106 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors.
Funding
Abstract
Retrograde axonal transport of nerve growth factor (NGF) signals is critical for the survival, differentiation, and maintenance of peripheral sympathetic and sensory neurons and basal forebrain cholinergic neurons. However, the mechanisms by which the NGF signal is propagated from the axon terminal to the cell body are yet to be fully elucidated. To gain insight into the mechanisms, we used quantum dot-labeled NGF (QD-NGF) to track the movement of NGF in real time in compartmentalized culture of rat dorsal root ganglion (DRG) neurons. Our studies showed that active transport of NGF within the axons was characterized by rapid, unidirectional movements interrupted by frequent pauses. Almost all movements were retrograde, but short-distance anterograde movements were occasionally observed. Surprisingly, quantitative analysis at the single molecule level demonstrated that the majority of NGF-containing endosomes contained only a single NGF dimer. Electron microscopic analysis of axonal vesicles carrying QD-NGF confirmed this finding. The majority of QD-NGF was found to localize in vesicles 50-150 nm in diameter with a single lumen and no visible intralumenal membranous components. Our findings point to the possibility that a single NGF dimer is sufficient to sustain signaling during retrograde axonal transport to the cell body.
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