ArticleThe Journal of cell biology2007
An in vitro nuclear disassembly system reveals a role for the RanGTPase system and microtubule-dependent steps in nuclear envelope breakdown.
Article in The Journal of cell biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
- Article
- Mechanobiology of the nucleus during the G2-M transition.Nucleus (Austin, Tex.) · 2024Review
- CHK1 controls zygote pronuclear envelope breakdown by regulating F-actin through interacting with MICAL3.EMBO reports · 2024Article
- How the Oocyte Nucleolus Is Turned into a Karyosphere: The Role of Heterochromatin and Structural Proteins.Journal of developmental biology · 2024Review
- A dedicated cytoplasmic container collects extrachromosomal DNA away from the mammalian nucleus.Molecular biology of the cell · 2023Article
- Fine-tuning cell organelle dynamics during mitosis by small GTPases.Frontiers of medicine · 2022Review
- Review
- Nucleus-Cytoskeleton Crosstalk During Mitotic Entry.Frontiers in cell and developmental biology · 2021Review
- PLK-1 promotes the merger of the parental genome into a single nucleus by triggering lamina disassembly.eLife · 2020Article
- Article
- Cytosolic calcium localization and dynamics during early endosperm development in the genus Agave (Asparagales, Asparagaceae).Protoplasma · 2019Article
- Chromosome segregation errors generate a diverse spectrum of simple and complex genomic rearrangements.Nature genetics · 2019Article
- The dynein adaptor Hook2 plays essential roles in mitotic progression and cytokinesis.The Journal of cell biology · 2019Article
- Reconstituting Nuclear and Chromosome Dynamics UsingCold Spring Harbor protocols · 2019Article
- Dissociation of membrane-chromatin contacts is required for proper chromosome segregation in mitosis.Molecular biology of the cell · 2019Article
- Nucleus Assembly and Import inCold Spring Harbor protocols · 2018Article
- The prolyl isomerase FKBP25 regulates microtubule polymerization impacting cell cycle progression and genomic stability.Nucleic acids research · 2018Article
- Mitotic Disassembly of Nuclear Pore Complexes Involves CDK1- and PLK1-Mediated Phosphorylation of Key Interconnecting Nucleoporins.Developmental cell · 2017Article
- Channel Nucleoporins Recruit PLK-1 to Nuclear Pore Complexes to Direct Nuclear Envelope Breakdown in C. elegans.Developmental cell · 2017Article
- Zap70 and downstream RanBP2 are required for the exact timing of the meiotic cell cycle in oocytes.Cell cycle (Georgetown, Tex.) · 2017Article
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2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During prophase, vertebrate cells disassemble their nuclear envelope (NE) in the process of NE breakdown (NEBD). We have established an in vitro assay that uses mitotic Xenopus laevis egg extracts and semipermeabilized somatic cells bearing a green fluorescent protein-tagged NE marker to study the molecular requirements underlying the dynamic changes of the NE during NEBD by live microscopy. We applied our in vitro system to analyze the role of the Ran guanosine triphosphatase (GTPase) system in NEBD. Our study shows that high levels of RanGTP affect the dynamics of late steps of NEBD in vitro. Also, inhibition of RanGTP production by RanT24N blocks the dynamic rupture of nuclei, suggesting that the local generation of RanGTP around chromatin may serve as a spatial cue in NEBD. Furthermore, the microtubule-depolymerizing drug nocodazole interferes with late steps of nuclear disassembly in vitro. High resolution live cell imaging reveals that microtubules are involved in the completion of NEBD in vivo by facilitating the efficient removal of membranes from chromatin.
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