ArticleJournal of virological methods2007
Multiplex real-time RT-PCR for the simultaneous detection and quantification of transmissible gastroenteritis virus and porcine epidemic diarrhea virus.
Article in Journal of virological methods, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 94 papers.
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Who cites it
94 citing papers in PubMed, 140 citations in OpenAlex.
- A monoclonal antibody-based electrochemical immunosensor for porcine epidemic diarrhea virus.Applied microbiology and biotechnology · 2026Article
- Isolation, genomic characterization, and pathogenicity of an emerging PEDV variant in Korea related to virulent Chinese strains.Frontiers in veterinary science · 2026Article
- Relevance of porcine intestinal organoids as a surrogate for animal experimentation: application to the investigation of host-virus interactions during porcine coronavirus infection.Veterinary research · 2025Article
- Advances in porcine epidemic diarrhea virus research: genome, epidemiology, vaccines, and detection methods.Discover nano · 2025Review
- Establishment and application of a quadruple RT-qPCR method for simultaneous detection of porcine enteric coronaviruses.Frontiers in veterinary science · 2025Article
- Pyrococcus furiosus Argonaute-mediated porcine epidemic diarrhea virus detection.Applied microbiology and biotechnology · 2024Article
- Establishment and Application of a Triplex Real-Time Reverse-Transcription Polymerase Chain Reaction Assay for Differentiation of PEDV, TGEV and PKV.Veterinary sciences · 2024Article
- Development of a CRISPR-Cas12a based assay for the detection of swine enteric coronaviruses in pig herds in China.Advanced biotechnology · 2024Article
- Detection ofMicroorganisms · 2024Article
- Development of a colloidal gold immunochromatographic strip for the simultaneous detection of porcine epidemic diarrhea virus and transmissible gastroenteritis virus.Frontiers in microbiology · 2024Article
- Article
- The impact and complete genome characterisation of viruses involved in outbreaks of gastroenteritis in a farrow-to-finish holding.Scientific reports · 2023Article
- A duplex nested RT-PCR method for monitoring porcine epidemic diarrhea virus and porcine delta-coronavirus.BMC veterinary research · 2023Article
- Multiplex Real-Time RT-PCR Assays for Detection and Differentiation of Porcine Enteric Coronaviruses.Pathogens (Basel, Switzerland) · 2023Article
- Article
- Development of a Multiplex Quantitative PCR for Detecting Porcine Epidemic Diarrhea Virus, Transmissible Gastroenteritis Virus, and Porcine Deltacoronavirus Simultaneously in China.Veterinary sciences · 2023Article
- Biological Characteristics and Pathogenicity Analysis of a Low Virulence G2a Porcine Epidemic Diarrhea Virus.Microbiology spectrum · 2023Article
- Experimental Infection of Pigs with Recent European Porcine Epidemic Diarrhea Viruses.Viruses · 2022Article
- A Quadruplex qRT-PCR for Differential Detection of Four Porcine Enteric Coronaviruses.Veterinary sciences · 2022Article
- Visual and Rapid Detection of Porcine Epidemic Diarrhea Virus (PEDV) Using Reverse Transcription Loop-Mediated Isothermal Amplification Method.Animals : an open access journal from MDPI · 2022Article
34 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transmissible gastroenteritis virus (TGEV) and porcine epidemic diarrhea virus (PEDV) are major etiological agents of diarrhea and death in piglets. Multiplex real-time reverse transcriptase (RT)-PCR was developed for simultaneous differential quantification of each virus in a single reaction tube, using Cy5- and FAM-labeled TaqMan-probes based on sequences from the TGEV and PEDV nucleocapsid genes. The copy numbers for transcripts of TGEV and PEDV were quantified using this assay over a range from 9x10(7) to 9x10(1) copies and 7x10(7) to 7x10(1) copies, respectively. The variability of the intra-assay and inter-assay were evaluated using standard solutions of each transcript, with coefficients of variation (CV) less than 3.43 and 3.33%, respectively. Piglets were experimentally infected with virulent TGEV and PEDV, and the amounts of virus from the onset of diarrhea were measured. Samples obtained from farms experiencing PED or TGE were quantified between 10(2) and 10(5) RNA copies. In conclusion, this assay provides an effective etiological diagnostic tool for detecting and quantifying viral loads. The assay may also prove useful for detecting infections, ultimately leading to better disease control on farms.
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