ArticleParasitology2007
Type I and type II fatty acid biosynthesis in Eimeria tenella: enoyl reductase activity and structure.
Article in Parasitology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- FromiScience · 2024Article
- Eimeria proteins: order amidst disorder.Parasites & vectors · 2022Review
- Fatty Acid Biosynthesis in Chromerids.Biomolecules · 2020Article
- Triclosan inhibits the growth of Neospora caninum in vitro and in vivo.Parasitology research · 2019Article
- The benzimidazole based drugs show good activity against T. gondii but poor activity against its proposed enoyl reductase enzyme target.Bioorganic & medicinal chemistry letters · 2014Article
- Discrimination of potent inhibitors of Toxoplasma gondii enoyl-acyl carrier protein reductase by a thermal shift assay.Biochemistry · 2013Article
- Lipid synthesis in protozoan parasites: a comparison between kinetoplastids and apicomplexans.Progress in lipid research · 2013Review
- Modification of triclosan scaffold in search of improved inhibitors for enoyl-acyl carrier protein (ACP) reductase in Toxoplasma gondii.ChemMedChem · 2013Article
- Development of a triclosan scaffold which allows for adaptations on both the A- and B-ring for transport peptides.Bioorganic & medicinal chemistry letters · 2013Article
- Design, synthesis, and biological activity of diaryl ether inhibitors of Toxoplasma gondii enoyl reductase.Bioorganic & medicinal chemistry letters · 2013Article
- High-resolution structures of Thermus thermophilus enoyl-acyl carrier protein reductase in the apo form, in complex with NAD+ and in complex with NAD+ and triclosan.Acta crystallographica. Section F, Structural biology and crystallization communications · 2012Article
- Characterization of acyl carrier protein and LytB in Babesia bovis apicoplast.Molecular and biochemical parasitology · 2012Article
- Lipoic acid metabolism of Plasmodium--a suitable drug target.Current pharmaceutical design · 2012Review
- The reductase domain in a Type I fatty acid synthase from the apicomplexan Cryptosporidium parvum: restricted substrate preference towards very long chain fatty acyl thioesters.BMC biochemistry · 2010Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
Abstract
Apicomplexan parasites of the genus Eimeria are the major causative agent of avian coccidiosis, leading to high economic losses in the poultry industry. Recent results show that Eimeria tenella harbours an apicoplast organelle, and that a key biosynthetic enzyme, enoyl reductase, is located in this organelle. In related parasites, enoyl reductase is one component of a type II fatty acid synthase (FAS) and has proven to be an attractive target for antimicrobial compounds. We cloned and expressed the mature form of E. tenella enoyl reductase (EtENR) for biochemical and structural studies. Recombinant EtENR exhibits NADH-dependent enoyl reductase activity and is inhibited by triclosan with an IC50 value of 60 nm. The crystal structure of EtENR reveals overall similarity with other ENR enzymes; however, the active site of EtENR is unoccupied, a state rarely observed in other ENR structures. Furthermore, the position of the central beta-sheet appears to block NADH binding and would require significant movement to allow NADH binding, a feature not previously seen in the ENR family. We analysed the E. tenella genomic database for orthologues of well-characterized bacterial and apicomplexan FAS enzymes and identified 6 additional genes, suggesting that E. tenella contains a type II FAS capable of synthesizing saturated, but not unsaturated, fatty acids. Interestingly, we also identified sequences that appear to encode multifunctional type I FAS enzymes, a feature also observed in Toxoplasma gondii, highlighting the similarity between these apicomplexan parasites.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.