ArticleMarine biotechnology (New York, N.Y.)
Phytoplankton-group specific quantitative polymerase chain reaction assays for RuBisCO mRNA transcripts in seawater.
Article in Marine biotechnology (New York, N.Y.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Toxin Dynamics among Populations of the Bioluminescent HAB SpeciesMarine drugs · 2024Article
- Ecological stoichiometry influences phytoplankton alpha and beta diversity rather than the community stability in subtropical bay.Ecology and evolution · 2022Article
- Revealing RNA virus diversity and evolution in unicellular algae transcriptomes.Virus evolution · 2021Article
- Distribution of Chromophytic Phytoplankton in the Eddy-Induced Upwelling Region of the West Pacific Ocean Revealed UsingFrontiers in microbiology · 2021Article
- Diversity and Spatial Distribution of Chromophytic Phytoplankton in the Bay of Bengal Revealed by RuBisCO Genes (Frontiers in microbiology · 2019Article
- Response of Spring Diatoms to CO2 Availability in the Western North Pacific as Determined by Next-Generation Sequencing.PloS one · 2016Article
- BioDry: An Inexpensive, Low-Power Method to Preserve Aquatic Microbial Biomass at Room Temperature.PloS one · 2015Article
- Clade-Specific Quantitative Analysis of Photosynthetic Gene Expression in Prochlorococcus.PloS one · 2015Article
- Quantification of diatom gene expression in the sea by selecting uniformly transcribed mRNA as the basis for normalization.Applied and environmental microbiology · 2012Article
- Diversity and expression of RubisCO genes in a perennially ice-covered Antarctic lake during the polar night transition.Applied and environmental microbiology · 2012Article
- Diversity of phytoplankton nitrate transporter sequences from isolated single cells and mixed samples from the East China Sea and mRNA quantification.Applied and environmental microbiology · 2011Article
- Use of inorganic and organic nitrogen by Synechococcus spp. and diatoms on the west Florida shelf as measured using stable isotope probing.Applied and environmental microbiology · 2009Article
- Quantification of diatom and dinoflagellate biomasses in coastal marine seawater samples by real-time PCR.Applied and environmental microbiology · 2008Article
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Authors and funding
3 authors.
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Abstract
The gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcL) has been shown to be a useful target for molecular assays that quantify form- or clade-specific RNA transcript concentrations as a proxy for the carbon fixation activity of marine phytoplankton. To improve the phylogenetic specificity and sensitivity of RNA probe hybridization methods, a quantitative reverse transcription-polymerase chain reaction (RT-PCR) assay has been reported for diatom and pelagophyte rbcL RNA. Here we detail enhancements made to this PCR method and development of additional assays to specifically quantify rbcL expression from haptophytes, Synechococcus and high-light Prochlorococcus. In vitro RNA transcripts were tested to demonstrate specificity and quantitative accuracy. Application of these methods on seawater samples from two depth profiles in the northern Gulf of Mexico showed a fair degree of agreement between PCR and hybridization results, with results for the chromophytic or form ID rbcL-containing organisms having better agreement between the two methods. Diatoms and other heterokonts were shown to be the primary carbon fixers at these locations by PCR, in agreement with greater form ID rbcL RNA measured by hybridization.
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