ArticleApplied and environmental microbiology2007
Shuffling of promoters for multiple genes to optimize xylose fermentation in an engineered Saccharomyces cerevisiae strain.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed, 90 citations in OpenAlex.
- EngineeringEngineering microbiology · 2024Review
- Genetic engineering of complex feed enzymes into barley seed for direct utilization in animal feedstuff.Plant biotechnology journal · 2023Article
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- Controlling gene expression with deep generative design of regulatory DNA.Nature communications · 2022Article
- Review
- Predictive design of sigma factor-specific promoters.Nature communications · 2020Article
- Bidirectional titration of yeast gene expression using a pooled CRISPR guide RNA approach.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- CRISPR-assisted multi-dimensional regulation for fine-tuning gene expression in Bacillus subtilis.Nucleic acids research · 2019Article
- Condition-specific promoter activities in Saccharomyces cerevisiae.Microbial cell factories · 2018Article
- Co-generation of ethanol and l-lactic acid from corn stalk under a hybrid process.Biotechnology for biofuels · 2018Article
- Rapid evolution of regulatory element libraries for tunable transcriptional and translational control of gene expression.Synthetic and systems biotechnology · 2017Article
- Genetic improvement of xylose metabolism by enhancing the expression of pentose phosphate pathway genes in Saccharomyces cerevisiae IR-2 for high-temperature ethanol production.Journal of industrial microbiology & biotechnology · 2017Article
- Enhanced xylose fermentation by engineered yeast expressing NADH oxidase through high cell density inoculums.Journal of industrial microbiology & biotechnology · 2017Article
- The application of powerful promoters to enhance gene expression in industrial microorganisms.World journal of microbiology & biotechnology · 2017Review
- High-throughput evaluation of synthetic metabolic pathways.Technology · 2016Article
- Article
- Bacterial xylose isomerases from the mammal gut Bacteroidetes cluster function in Saccharomyces cerevisiae for effective xylose fermentation.Microbial cell factories · 2015Article
- Combinatorial Screening for Transgenic Yeasts with High Cellulase Activities in Combination with a Tunable Expression System.PloS one · 2015Article
- Transcription interference and ORF nature strongly affect promoter strength in a reconstituted metabolic pathway.Frontiers in bioengineering and biotechnology · 2015Article
- Heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in Saccharomyces cerevisiae.Frontiers in microbiology · 2015Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
We describe here a useful metabolic engineering tool, multiple-gene-promoter shuffling (MGPS), to optimize expression levels for multiple genes. This method approaches an optimized gene overexpression level by fusing promoters of various strengths to genes of interest for a particular pathway. Selection of these promoters is based on the expression levels of the native genes under the same physiological conditions intended for the application. MGPS was implemented in a yeast xylose fermentation mixture by shuffling the promoters for GND2 and HXK2 with the genes for transaldolase (TAL1), transketolase (TKL1), and pyruvate kinase (PYK1) in the Saccharomyces cerevisiae strain FPL-YSX3. This host strain has integrated xylose-metabolizing genes, including xylose reductase, xylitol dehydrogenase, and xylulose kinase. The optimal expression levels for TAL1, TKL1, and PYK1 were identified by analysis of volumetric ethanol production by transformed cells. We found the optimal combination for ethanol production to be GND2-TAL1-HXK2-TKL1-HXK2-PYK1. The MGPS method could easily be adapted for other eukaryotic and prokaryotic organisms to optimize expression of genes for industrial fermentation.
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