ArticleProceedings of the National Academy of Sciences of the United States of America2007
Bacterial sensor kinase TodS interacts with agonistic and antagonistic signals.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Chemotaxis to plant defense compounds in phytopathogens.PLoS pathogens · 2026Article
- Advances in ligand-specific biosensing for structurally similar molecules.Cell systems · 2023Review
- Genetic Analysis Reveals a Requirement for the Hybrid Sensor Kinase RscS inJournal of bacteriology · 2023Article
- Article
- Fluoro-recognition: New in vivo fluorescent assay for toluene dioxygenase probing induction by and metabolism of polyfluorinated compounds.Environmental microbiology · 2022Article
- Antimicrobial resistance: progress and challenges in antibiotic discovery and anti-infective therapy.Microbial biotechnology · 2022Article
- Flagella, Chemotaxis and Surface Sensing.Advances in experimental medicine and biology · 2022Article
- Diversity in Sensing and Signaling of Bacterial Sensor Histidine Kinases.Biomolecules · 2021Review
- Article
- The Evanescent GacS Signal.Microorganisms · 2020Review
- Characterisation of sensor kinase by CD spectroscopy: golden rules and tips.Biochemical Society transactions · 2018Review
- The activity of the C4-dicarboxylic acid chemoreceptor of Pseudomonas aeruginosa is controlled by chemoattractants and antagonists.Scientific reports · 2018Article
- Histidine kinases and the missing phosphoproteome from prokaryotes to eukaryotes.Laboratory investigation; a journal of technical methods and pathology · 2018Review
- Lvr, a Signaling System That Controls Global Gene Regulation and Virulence in PathogenicFrontiers in cellular and infection microbiology · 2018Article
- Transcriptional Regulation of the Peripheral Pathway for the Anaerobic Catabolism of Toluene andFrontiers in microbiology · 2018Article
- Metabolic Value Chemoattractants Are Preferentially Recognized at Broad Ligand Range Chemoreceptor ofFrontiers in microbiology · 2017Article
- Degradation of cyclic diguanosine monophosphate by a hybrid two-component protein protects Azoarcus sp. strain CIB from toluene toxicity.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- New family of biosensors for monitoring BTX in aquatic and edaphic environments.Microbial biotechnology · 2016Article
- Molecular Insights into Toluene Sensing in the TodS/TodT Signal Transduction System.The Journal of biological chemistry · 2016Article
- Multiple signals modulate the activity of the complex sensor kinase TodS.Microbial biotechnology · 2015Article
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5 authors.
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Abstract
The TodS/TodT two-component system controls expression of the toluene dioxygenase (TOD) pathway for the metabolism of toluene in Pseudomonas putida DOT-T1E. TodS is a sensor kinase that ultimately controls tod gene expression through its cognate response regulator, TodT. We used isothermal titration calorimetry to study the binding of different compounds to TodS and related these findings to their capacity to induce gene expression in vivo. Agonistic compounds bound to TodS and induced gene expression in vivo. Toluene was a powerful agonist, but ortho-substitutions of toluene reduced or abolished in vivo responses, although TodS recognized o-xylene with high affinity. These compounds were called antagonists. We show that agonists and antagonists compete for binding to TodS both in vitro and in vivo. The failure of antagonists to induce gene expression in vivo correlated with their inability to stimulate TodS autophosphorylation in vitro. We propose intramolecular TodS signal transmission, not molecular recognition of compounds by TodS, to be the phenomenon that determines whether a given compound will lead to activation of expression of the tod genes. Molecular modeling identified residues F46, I74, F79, and I114 as being potentially involved in the binding of effector molecules. Alanine substitution mutants of these residues reduced affinities (2- to 345-fold) for both agonistic and antagonistic compounds. Our data indicate that determining the inhibitory activity of antagonists is a potentially fruitful alternative to design specific two-component system inhibitors for the development of new drugs to inhibit processes regulated by two-component systems.
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