ArticlePlant physiology2007
Generation of single-copy T-DNA transformants in Arabidopsis by the CRE/loxP recombination-mediated resolution system.
Article in Plant physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 48 citations in OpenAlex.
- Unclasping potentials of genomics and gene editing in chickpea to fight climate change and global hunger threat.Frontiers in genetics · 2023Review
- IMPLANT: a new technique for transgene copy number estimation in plants using a single end-point PCR reaction.Plant methods · 2022Article
- Optimized Transformation and Gene Editing of the B104 Public Maize Inbred by Improved Tissue Culture and Use of Morphogenic Regulators.Frontiers in plant science · 2022Article
- Generation of a selectable marker free, highly expressed single copy locus as landing pad for transgene stacking in sugarcane.Plant molecular biology · 2019Article
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- Induction of Terpene Biosynthesis in Berries of Microvine Transformed withFrontiers in plant science · 2017Article
- Gene stacking by recombinases.Plant biotechnology journal · 2016Review
- A Tet-on and Cre-loxP Based Genetic Engineering System for Convenient Recycling of Selection Markers in Penicillium oxalicum.Frontiers in microbiology · 2016Article
- Article
- Enhanced transgene expression in rice following selection controlled by weak promoters.BMC biotechnology · 2013Article
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- Genetic rearrangements can modify chromatin features at epialleles.PLoS genetics · 2011Article
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- Transgene excision from wheat chromosomes by phage phiC31 integrase.Plant molecular biology · 2010Article
- Transcriptome analysis reveals absence of unintended effects in drought-tolerant transgenic plants overexpressing the transcription factor ABF3.BMC genomics · 2010Article
- Evaluation of seven promoters to achieve germline directed Cre-lox recombination in Arabidopsis thaliana.Plant cell reports · 2009Article
- Simultaneous excision of two transgene flanking sequences and resolution of complex integration loci.Plant molecular biology · 2009Article
- Expression of active Streptomyces phage phiC31 integrase in transgenic wheat plants.Plant cell reports · 2008Article
- Advanced expression vector systems: new weapons for plant research and biotechnology.Plant physiology · 2007Article
- Marker-free transgenic plants through genetically programmed auto-excision.Plant physiology · 2007Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated whether complex T-DNA loci, often resulting in low transgene expression, can be resolved efficiently into single copies by CRE/loxP-mediated recombination. An SB-loxP T-DNA, containing two invertedly oriented loxP sequences located inside and immediately adjacent to the T-DNA border ends, was constructed. Regardless of the orientation and number of SB-loxP-derived T-DNAs integrated at one locus, recombination between the outermost loxP sequences in direct orientation should resolve multiple copies into a single T-DNA copy. Seven transformants with a complex SB-loxP locus were crossed with a CRE-expressing plant. In three hybrids, the complex T-DNA locus was reduced efficiently to a single-copy locus. Upon segregation of the CRE recombinase gene, only the simplified T-DNA locus was found in the progeny, demonstrating DNA had been excised efficiently in the progenitor cells of the gametes. In the two transformants with an inverted T-DNA repeat, the T-DNA resolution was accompanied by at least a 10-fold enhanced transgene expression. Therefore, the resolution of complex loci to a single-copy T-DNA insert by the CRE/loxP recombination system can become a valuable method for the production of elite transgenic Arabidopsis thaliana plants that are less prone to gene silencing.
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