ArticleThe Plant cell2007
Targeted degradation of PSEUDO-RESPONSE REGULATOR5 by an SCFZTL complex regulates clock function and photomorphogenesis in Arabidopsis thaliana.
Article in The Plant cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 135 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
135 citing papers in PubMed, 1 synthesis or guideline pooled it, 264 citations in OpenAlex.
- Shades of green: untying the knots of green photoperception.Journal of experimental botany · 2020Pooled it
- CCRG1 Acts as a Downstream Effector of the Clock Proteins CCA1 and LHY to Coordinate Growth, Flowering, and Senescence.Plant, cell & environment · 2026Article
- Unveiling previously undescribed circadian clock regulators and action mechanisms via TurboID-based profiling of the LWD1 interactome inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Light at the heart of time: How photoreceptors entrain the circadian clock.Plant communications · 2026Review
- Bioinformatics mining and clinical validation of CD274 as a prognostic indicator in hepatocellular carcinoma.BMC cancer · 2026Article
- Rice OsFKF1 Promotes Degradation of OsPRR37 for Short-Day Early Flowering.Rice (New York, N.Y.) · 2025Article
- Dissecting usuranme biquitin-mediated circadian clock regulation in Arabidopsis via high-throughput yeast screens.Journal of experimental botany · 2025Article
- Temperature compensation of the Arabidopsis circadian clock.Npj biological timing and sleep · 2025Review
- Ectopic Over-Expression ofPlants (Basel, Switzerland) · 2025Article
- The circadian clock of Populus affects physiological, transcriptional and metabolomic responses to osmotic and ionic components of salt stress.Npj biological timing and sleep · 2025Article
- Abundant clock proteins point to missing molecular regulation in the plant circadian clock.Molecular systems biology · 2025Article
- Nature's Master of Ceremony: TheNpj biological timing and sleep · 2025Article
- HOS15-mediated turnover of PRR7 enhances freezing tolerance.The New phytologist · 2024Article
- Forward genetic approach identifies a phylogenetically conserved serine residue critical for the catalytic activity of UBIQUITIN-SPECIFIC PROTEASE 12 in Arabidopsis.Scientific reports · 2024Article
- LIP1 Regulates the Plant Circadian Oscillator by Modulating the Function of the Clock Component GIGANTEA.Cells · 2024Article
- Agrobacterium-mediated transformation of B. juncea reveals that BjuLKP2 functions in plant yellowing.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- 40S Ribosomal protein S6 kinase integrates daylength perception and growth regulation in Arabidopsis thaliana.Plant physiology · 2024Article
- HOS15-mediated turnover of PRR7 enhances freezing tolerance.bioRxiv : the preprint server for biology · 2024Article
- Circadian and photoperiodic regulation of the vegetative to reproductive transition in plants.Communications biology · 2024Review
- Multi-regulated GDP-l-galactose phosphorylase calls the tune in ascorbate biosynthesis.Journal of experimental botany · 2024Review
75 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Circadian clocks comprise several regulatory feedback loops that control gene transcription. However, recent evidence has shown that posttranslational mechanisms are also required for clock function. In Arabidopsis thaliana, members of the PSEUDO-RESPONSE REGULATOR (PRR) family were proposed to be components of the central oscillator. Using a PRR5-specific antibody, we characterized changes in PRR5 protein levels in relation to its mRNA levels under various circadian conditions. Under long-day conditions, PRR5 mRNA levels are undetectable at dusk but PRR5 protein levels remain maximal. Upon dark transition, however, PRR5 levels decrease rapidly, indicating dark-induced, posttranslational regulation. We demonstrated that the Pseudo-Receiver (PR) domain of PRR5 interacts directly with the F box protein ZEITLUPE (ZTL) in vitro and in vivo. Analyses of mutants and transgenic plants revealed an inverse correlation between PRR5 and ZTL levels, which depends on the PR domain. These results indicate that PRR5 is negatively regulated by ZTL, which likely mediates its ubiquitination and degradation. Phenotypic analyses of prr5 ztl double mutants showed that PRR5 is required for ZTL functions. ZTL contains a Light-Oxygen-Voltage domain, and its activity may be directly regulated by blue light. Consistent with this notion, we found that blue light stabilizes PRR5, although it does not alter ZTL levels. Together, our results show that ZTL targets PRR5 for degradation by 26S proteasomes in the circadian clock and in early photomorphogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.