ArticleJournal of bacteriology2007
Characterization of riboflavin (vitamin B2) transport proteins from Bacillus subtilis and Corynebacterium glutamicum.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
65 citing papers in PubMed, 117 citations in OpenAlex.
- Purine permease 5 contributes to riboflavin distribution in Arabidopsis reproductive organs.Plant & cell physiology · 2026Article
- Shedding light on YfhS and YjlC: novel effectors of the NADH dehydrogenase activity of the electron transport chain inbioRxiv : the preprint server for biology · 2026Article
- Effect of riboflavin and roseoflavin, a structural analogue of riboflavin, on the growth of selected microbial isolates from human saliva using a riboflavin-free cultivation medium.Folia microbiologica · 2025Article
- Vitamin biosynthesis in the gut: interplay between mammalian host and its resident microbiota.Microbiology and molecular biology reviews : MMBR · 2025Review
- The current riboswitch landscape inMicrobiology (Reading, England) · 2024Review
- Isolation, characterisation and description of the roseoflavin producer Streptomyces berlinensis sp. nov.Environmental microbiology reports · 2024Article
- The coenzyme BMicrobial cell (Graz, Austria) · 2023Article
- Metabolic engineering of thermophilic Bacillus methanolicus for riboflavin overproduction from methanol.Microbial biotechnology · 2023Article
- Functional comparison of metabolic networks across species.Nature communications · 2023Article
- Postbiotics in rheumatoid arthritis: emerging mechanisms and intervention perspectives.Frontiers in microbiology · 2023Review
- Article
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- Engineering cofactor supply and recycling to drive phenolic acid biosynthesis in yeast.Nature chemical biology · 2022Article
- A second riboswitch class for the enzyme cofactor NADRNA (New York, N.Y.) · 2021Article
- Characterization of the Streptococcus mutansJournal of bacteriology · 2020Article
- Benefit of Dietary Supplementation with Bacillus subtilis BYS2 on Growth Performance, Immune Response, and Disease Resistance of Broilers.Probiotics and antimicrobial proteins · 2020Article
- Molecular Elucidation of Riboflavin Production and Regulation in Candida albicans, toward a Novel Antifungal Drug Target.mSphere · 2020Article
- Methanol-Essential Growth ofInternational journal of molecular sciences · 2020Article
- Structural and Functional Characterization of NadR fromMolecules (Basel, Switzerland) · 2020Article
- Simultaneous and sequential based co-fermentations of Trichoderma asperellum GDFS1009 and Bacillus amyloliquefaciens 1841: a strategy to enhance the gene expression and metabolites to improve the bio-control and plant growth promoting activity.Microbial cell factories · 2019Article
5 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Riboflavin (vitamin B(2)) is the direct precursor of the flavin cofactors flavin mononucleotide and flavin adenine dinucleotide, essential components of cellular biochemistry. In this work we investigated the unrelated proteins YpaA from Bacillus subtilis and PnuX from Corynebacterium glutamicum for a role in riboflavin uptake. Based on the regulation of the corresponding genes by a riboswitch mechanism, both proteins have been predicted to be involved in flavin metabolism. Moreover, their primary structures suggested that these proteins integrate into the cytoplasmic membrane. We provide experimental evidence that YpaA is a plasma membrane protein with five transmembrane domains and a cytoplasmic C terminus. In B. subtilis, riboflavin uptake was increased when ypaA was overexpressed and abolished when ypaA was deleted. Riboflavin uptake activity and the abundance of the YpaA protein were also increased when riboflavin auxotrophic mutants were grown in limiting amounts of riboflavin. YpaA-mediated riboflavin uptake was sensitive to protonophors and reduced in the absence of glucose, demonstrating that the protein requires metabolic energy for substrate translocation. In addition, we demonstrate that PnuX from C. glutamicum also is a riboflavin transporter. Transport by PnuX was not energy dependent and had high apparent affinity for riboflavin (K(m) 11 microM). Roseoflavin, a toxic riboflavin analog, appears to be a substrate of PnuX and YpaA. We propose to designate the gene names ribU for ypaA and ribM for pnuX to reflect that the encoded proteins function in riboflavin uptake and that the genes have different phylogenetic origins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.