ArticleBiophysical journal2007
Structure and phase diagram of nucleosome core particles aggregated by multivalent cations.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 77 citations in OpenAlex.
- Histone H3 tail charge patterns govern nucleosome condensate formation and dynamics.Nucleic acids research · 2026Article
- Article
- Histone H3 tail charge patterns govern nucleosome condensate formation and dynamics.bioRxiv : the preprint server for biology · 2025Article
- The impact of charge regulation and ionic intranuclear environment on the nucleosome core particle.The Journal of chemical physics · 2024Article
- The Impact of Charge Regulation and Ionic Intranuclear Environment on the Nucleosome Core Particle.bioRxiv : the preprint server for biology · 2024Article
- Multiscale modeling reveals the ion-mediated phase separation of nucleosome core particles.Biophysical journal · 2024Article
- Structural and dynamical investigation of histone H2B in well-hydrated nucleosome core particles by solid-state NMR.Communications biology · 2023Article
- A Bottom-Up Coarse-Grained Model for Nucleosome-Nucleosome Interactions with Explicit Ions.Journal of chemical theory and computation · 2022Article
- Histone H3 and H4 tails play an important role in nucleosome phase separation.Biophysical chemistry · 2022Article
- DNA origami nano-mechanics.Chemical Society reviews · 2021Review
- De novo reconstitution of chromatin using wheat germ cell-free protein synthesis.FEBS open bio · 2021Article
- Characterization of nucleosome sediments for protein interaction studies by solid-state NMR spectroscopy.Magnetic resonance (Gottingen, Germany) · 2021Article
- Frozen-hydrated chromatin from metaphase chromosomes has an interdigitated multilayer structure.The EMBO journal · 2019Article
- Nucleosome conformational variability in solution and in interphase nuclei evidenced by cryo-electron microscopy of vitreous sections.Nucleic acids research · 2018Article
- Site-Specific Studies of Nucleosome Interactions by Solid-State NMR Spectroscopy.Angewandte Chemie (International ed. in English) · 2018Article
- A systematic analysis of nucleosome core particle and nucleosome-nucleosome stacking structure.Scientific reports · 2018Article
- Article
- Uncovering the forces between nucleosomes using DNA origami.Science advances · 2016Article
- The Influence of Ionic Environment and Histone Tails on Columnar Order of Nucleosome Core Particles.Biophysical journal · 2016Article
- Crop epigenetics and the molecular hardware of genotype × environment interactions.Frontiers in plant science · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The degree of compaction of the eukaryotic chromatin in vivo and in vitro is highly sensitive to the ionic environment. We address the question of the effect of multivalent ions on the interactions and mutual organization of the chromatin structural units, the nucleosome core particles (NCPs). Conditions of precipitation of NCPs in the presence of 10 mM Tris buffer and various amounts of either magnesium (Mg(2+)) or spermidine (Spd(3+)) are explored, compared, and discussed in relation to theoretical models. In addition, the structure of the aggregates is analyzed by complementary techniques: freeze-fracture electron microscopy, cryoelectron microscopy, and x-ray diffraction. In Mg(2+)-NCP aggregates, NCPs tend to stack on top of one another to form columns that are not long-range organized. In the presence of Spd(3+), NCPs precipitate to form a dense isotropic phase, a disordered phase of columns, a two-dimensional columnar hexagonal phase, or a three-dimensional crystal. The more ordered phases (two-dimensional or three-dimensional hexagonal) are found close to the precipitation line, where the number of positive charges carried by cations is slightly larger than the number of available negative charges of the NCPs. All ordered phases coexist with the dense isotropic phases. Formation of hexagonal and columnar phases is prevented by an excess of polycations.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.