ArticleProceedings of the National Academy of Sciences of the United States of America2007
The tight junction protein, MUPP1, is up-regulated by hypertonicity and is important in the osmotic stress response in kidney cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 50 citations in OpenAlex.
- Identification of a common ketohexokinase-dependent link driving alcohol intake and alcohol-associated liver disease in mice.Nature metabolism · 2025Article
- Investigating the role of gut microbiota in diabetic nephropathy through plasma proteome mediated analysis.Scientific reports · 2025Article
- Integrating spatial transcriptomics with single-cell transcriptomics reveals a spatiotemporal gene landscape of the human developing kidney.Cell & bioscience · 2022Article
- Umami-induced obesity and metabolic syndrome is mediated by nucleotide degradation and uric acid generation.Nature metabolism · 2021Article
- MPDZ as a novel epigenetic silenced tumor suppressor inhibits growth and progression of lung cancer through the Hippo-YAP pathway.Oncogene · 2021Article
- Vasopressin mediates fructose-induced metabolic syndrome by activating the V1b receptor.JCI insight · 2021Article
- Sugar causes obesity and metabolic syndrome in mice independently of sweet taste.American journal of physiology. Endocrinology and metabolism · 2020Article
- Deletion of Fructokinase in the Liver or in the Intestine Reveals Differential Effects on Sugar-Induced Metabolic Dysfunction.Cell metabolism · 2020Article
- Claudin 19 Is Regulated by Extracellular Osmolality in Rat Kidney Inner Medullary Collecting Duct Cells.International journal of molecular sciences · 2019Article
- Ketohexokinase C blockade ameliorates fructose-induced metabolic dysfunction in fructose-sensitive mice.The Journal of clinical investigation · 2018Article
- Copy number variations and expression of MPDZ are prognostic biomarkers for clear cell renal cell carcinoma.Oncotarget · 2017Article
- Effects of exogenous desmopressin on a model of heat stress nephropathy in mice.American journal of physiology. Renal physiology · 2017Article
- Protective role of fructokinase blockade in the pathogenesis of acute kidney injury in mice.Nature communications · 2017Article
- Aging-associated renal disease in mice is fructokinase dependent.American journal of physiology. Renal physiology · 2016Article
- Article
- Endogenous fructose production and metabolism in the liver contributes to the development of metabolic syndrome.Nature communications · 2013Article
- The role of hyperosmotic stress in inflammation and disease.Biomolecular concepts · 2012Article
- Sucrose induces fatty liver and pancreatic inflammation in male breeder rats independent of excess energy intake.Metabolism: clinical and experimental · 2011Article
- Effects of 2-bromoethanamine on TonEBP expression and its possible role in induction of renal papillary necrosis in mice.Toxicological sciences : an official journal of the Society of Toxicology · 2010Article
- The expression of aquaporin-1 in the medulla of the kidney is dependent on the transcription factor associated with hypertonicity, TonEBP.The Journal of biological chemistry · 2010Article
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6 authors at 2 institutions in 1 country.
Funding
Abstract
Antibody array proteomics was used to detect differentially expressed proteins in inner medullary collecting duct 3 (IMCD3) cells grown under isotonic and chronic hypertonic conditions. Of 512 potential proteins, >90% were unchanged in expression. Noteworthy was the up-regulation of several tight junction-related proteins, including MUPP1 (multi-PDZ protein-1), ZO1 (zonula occludens 1), and Af6. The most robustly up-regulated protein under hypertonic conditions was MUPP1 (7.2x, P < 0.001). Changes in expression for MUPP1 were verified by quantitative PCR for message and Western blot for protein. In mouse kidney tissues, MUPP1 expression was substantial in the papilla and was absent in the cortex. Furthermore, MUPP1 expression increased 253% (P < 0.01) in the papilla upon 36 h of thirsting. Localization of MUPP1 protein expression was confirmed by immunocytochemical analysis demonstrating only minor staining under isotonic conditions and the substantial presence in chronically adapted cells at the basolateral membrane. Message and protein half-life in IMCD3 cells were 26.2 and 17.8 h, respectively. Osmotic initiators of MUPP1 expression included NaCl, sucrose, mannitol, sodium acetate, and choline chloride but not urea. Stable IMCD3 clones silenced for MUPP1 expression used the pSM2-MUPP1 vector. In cell viability experiments, clones silenced for MUPP1 demonstrated only a minor loss in cell survival under acute sublethal osmotic stress compared with empty vector control cells. In contrast, a 24% loss (P < 0.02) in transepithelial resistance for monolayers of MUPP1-silenced cells was determined as compared with controls. These results suggest that MUPP1 specifically, and potentially tight junction complexes in general, are important in the renal osmoadaptive response.
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