ArticleThe Journal of molecular diagnostics : JMD2007
Rapid identification of promoter hypermethylation in hepatocellular carcinoma by pyrosequencing of etiologically homogeneous sample pools.
Article in The Journal of molecular diagnostics : JMD, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- Trial
- Is Stress Reactivity an Intermediary Mechanism in the Association Between NR3C1 Methylation and Adolescent Loneliness?Developmental psychobiology · 2026Article
- Early Diagnosis of Colorectal Cancer Based on Bisulfite-free Site-specific Methylation Identification PCR Strategy: High-Sensitivity, Accuracy, and Primary Medical Accessibility.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Identification and validation ofOncology letters · 2024Article
- KISS-1, Mediated by Promoter Methylation, Suppresses Esophageal Squamous Cell Carcinoma Metastasis via MMP2/9/MAPK Axis.Digestive diseases and sciences · 2022Article
- A novel method for early detection of colorectal cancer based on detection of methylation of two fragments of syndecan-2 (SDC2) in stool DNA.BMC gastroenterology · 2022Article
- Bisulfite profiling of the MGMT promoter and comparison with routine testing in glioblastoma diagnostics.Clinical epigenetics · 2022Article
- Maternal anxiety and depression in pregnancy and DNA methylation of theEpigenomics · 2021Article
- Reliable Clinical MLH1 Promoter Hypermethylation Assessment Using a High-Throughput Genome-Wide Methylation Array Platform.The Journal of molecular diagnostics : JMD · 2020Article
- Cell-free plasma hypermethylated CASZ1, CDH13 and ING2 are promising biomarkers of esophageal cancer.Journal of biomedical research · 2018Article
- Clinical cancer research : an official journal of the American Association for Cancer Research · 2018Article
- Feasibility of quantifyingClinical epigenetics · 2017Article
- DNA methylation biomarkers: cancer and beyond.Genes · 2014Review
- DNA methyltransferases 1 and 3b expression in Huh-7 cells expressing HCV core protein of different genotypes.Digestive diseases and sciences · 2012Article
- DNA methylation in peripheral blood: a potential biomarker for cancer molecular epidemiology.Journal of epidemiology · 2012Review
- Identification of type 1 diabetes-associated DNA methylation variable positions that precede disease diagnosis.PLoS genetics · 2011Article
- Precision of pyrosequencing assay to measure LINE-1 methylation in colon cancer, normal colonic mucosa, and peripheral blood cells.The Journal of molecular diagnostics : JMD · 2010Article
- Bisulfite-based epityping on pooled genomic DNA provides an accurate estimate of average group DNA methylation.Epigenetics & chromatin · 2009Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aberrant DNA methylation patterns have been identified in a variety of human diseases, particularly cancer. Pyrosequencing has evolved in recent years as a sensitive and accurate method for the analysis and quantification of the degree of DNA methylation in specific target regions. However, the number of candidate genes that can be analyzed in clinical specimens is often restricted by the limited amount of sample available. Here, we present a novel screening approach that enables the rapid identification of differentially methylated regions such as promoters by pyrosequencing of etiologically homogeneous sample pools after bisulfite treatment. We exemplify its use by the analysis of five genes (CDKN2A, GSTP1, MLH1, IGF2, and CTNNB1) involved in the pathogenesis of human hepatocellular carcinoma using pools stratified for different parameters of clinical importance. Results were confirmed by the individual analysis of the samples. The screening identified all genes displaying differential methylation successfully, and no false positives occurred. Quantitative comparison of the pools and the samples in the pool analyzed individually showed a deviation of approximately 1.5%, making the method ideally suited for the identification of diagnostic markers based on DNA methylation while saving precious DNA material.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.