ArticleMolecular biology of the cell2007
Abl tyrosine kinase promotes dorsal ruffles but restrains lamellipodia extension during cell spreading on fibronectin.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 25 citations in OpenAlex.
- c-Abl kinase at the crossroads of healthy synaptic remodeling and synaptic dysfunction in neurodegenerative diseases.Neural regeneration research · 2023Review
- Role of the ABL tyrosine kinases in the epithelial-mesenchymal transition and the metastatic cascade.Cell communication and signaling : CCS · 2021Review
- G-Protein GαJournal of molecular biology · 2017Article
- Reciprocal stabilization of ABL and TAZ regulates osteoblastogenesis through transcription factor RUNX2.The Journal of clinical investigation · 2016Article
- Redox control of Cas phosphorylation requires Abl kinase in regulation of intestinal epithelial cell spreading and migration.American journal of physiology. Gastrointestinal and liver physiology · 2016Article
- Role played by paxillin and paxillin tyrosine phosphorylation in hepatocyte growth factor/sphingosine-1-phosphate-mediated reactive oxygen species generation, lamellipodia formation, and endothelial barrier function.Pulmonary circulation · 2015Article
- Article
- Supervillin binds the Rac/Rho-GEF Trio and increases Trio-mediated Rac1 activation.Cytoskeleton (Hoboken, N.J.) · 2015Article
- ABL tyrosine kinase inhibition variable effects on the invasive properties of different triple negative breast cancer cell lines.PloS one · 2015Article
- Fluorescence lifetime imaging of biosensor peptide phosphorylation in single live cells.Angewandte Chemie (International ed. in English) · 2013Article
- Article
- Gleevec, an Abl family inhibitor, produces a profound change in cell shape and migration.PloS one · 2013Article
- Activation of abl family kinases in solid tumors.Genes & cancer · 2012Article
- 3BP2-deficient mice are osteoporotic with impaired osteoblast and osteoclast functions.The Journal of clinical investigation · 2011Article
- A peptide biosensor for detecting intracellular Abl kinase activity using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.Analytical biochemistry · 2010Article
- Regulation of cell migration and morphogenesis by Abl-family kinases: emerging mechanisms and physiological contexts.Journal of cell science · 2009Article
- 14-3-3beta-Rac1-p21 activated kinase signaling regulates Akt1-mediated cytoskeletal organization, lamellipodia formation and fibronectin matrix assembly.Journal of cellular physiology · 2009Article
- Induction of cell retraction by the combined actions of Abl-CrkII and Rho-ROCK1 signaling.The Journal of cell biology · 2008Article
- A crucial role in cell spreading for the interaction of Abl PxxP motifs with Crk and Nck adaptors.Journal of cell science · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The nonreceptor Abl tyrosine kinase stimulates F-actin microspikes and membrane ruffles in response to adhesion and growth factor signals. We show here that induced dimerization of Abl-FKBP, but not the kinase-defective AblKD-FKBP, inhibits cell spreading on fibronectin. Conversely, knockdown of cellular Abl by shRNA stimulates cell spreading. The Abl kinase inhibitor, imatinib, also stimulates cell spreading and its effect is overridden by the imatinib-resistant AblT315I. Expression of Abl but not AbkKD in Abl/Arg-deficient cells again inhibits spreading. Furthermore, Abl inhibits spreading of cells that express the activated Rac, RacV12, correlating with RacV12 localization to dorsal membrane protrusions. Ectopic expression of CrkII, a Rac activator that is inactivated by Abl-mediated tyrosine phosphorylation, antagonizes Abl-mediated dorsal membrane localization of RacV12. Ectopic expression of a dynamin-2 mutant, previously shown to induce Rac-GTP localization to the dorsal membrane, abolishes the stimulatory effect of imatinib on cell spreading. These results suggest that Abl tyrosine kinase, through CrkII phosphorylation and in collaboration with dynamin-2 can regulate the partitioning of Rac-GTP to favor dorsal ruffles during cell spreading. The Abl-dependent dorsal membrane localization of activated Rac explains its positive role in ruffling and negative role in cell spreading and migration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.