ArticleMolecular biology of the cell2007
Exocyst requirement for endocytic traffic directed toward the apical and basolateral poles of polarized MDCK cells.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
83 citing papers in PubMed, 124 citations in OpenAlex.
- The exocyst in ciliogenesis.Renal failure · 2025Review
- The exocyst complex is required for the trafficking and delivery of KCa3.1 to the basolateral membrane of polarized epithelia.American journal of physiology. Cell physiology · 2023Article
- The exocyst complex is an essential component of the mammalian constitutive secretory pathway.The Journal of cell biology · 2023Article
- Plasmolipin regulates basolateral-to-apical transcytosis of ICAM-1 and leukocyte adhesion in polarized hepatic epithelial cells.Cellular and molecular life sciences : CMLS · 2022Article
- RASSF8-mediated transport of Echinoid via the exocyst promotes Drosophila wing elongation and epithelial ordering.Development (Cambridge, England) · 2021Article
- Spatial bias in cAMP generation determines biological responses to PTH type 1 receptor activation.Science signaling · 2021Article
- Loss of the exocyst complex component EXOC3 promotes hemostasis and accelerates arterial thrombosis.Blood advances · 2021Article
- Epigenome-wide association study on diffusing capacity of the lung.ERJ open research · 2021Article
- Article
- Exocyst Genes Are Essential for Recycling Membrane Proteins and Maintaining Slit Diaphragm inJournal of the American Society of Nephrology : JASN · 2020Article
- The role of Exo70 in exocytosis and beyond.Small GTPases · 2019Review
- The exocyst controls lysosome secretion and antigen extraction at the immune synapse of B cells.The Journal of cell biology · 2019Article
- Mucosal absorption of therapeutic peptides by harnessing the endogenous sorting of glycosphingolipids.eLife · 2018Article
- Regulation of Cell Polarity by Exocyst-Mediated Trafficking.Cold Spring Harbor perspectives in biology · 2018Review
- The Structure and Biological Function of CREG.Frontiers in cell and developmental biology · 2018Review
- Polarized Exocytosis.Cold Spring Harbor perspectives in biology · 2017Review
- Cytoplasmic cleavage of DPPA3 is required for intracellular trafficking and cleavage-stage development in mice.Nature communications · 2017Article
- A targeted RNAi screen identifies factors affecting diverse stages of receptor-mediated transcytosis.The Journal of cell biology · 2017Article
- Article
- Septins promote macropinosome maturation and traffic to the lysosome by facilitating membrane fusion.The Journal of cell biology · 2016Article
23 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 5 institutions in 1 country.
Funding
Abstract
The octameric exocyst complex is associated with the junctional complex and recycling endosomes and is proposed to selectively tether cargo vesicles directed toward the basolateral surface of polarized Madin-Darby canine kidney (MDCK) cells. We observed that the exocyst subunits Sec6, Sec8, and Exo70 were localized to early endosomes, transferrin-positive common recycling endosomes, and Rab11a-positive apical recycling endosomes of polarized MDCK cells. Consistent with its localization to multiple populations of endosomes, addition of function-blocking Sec8 antibodies to streptolysin-O-permeabilized cells revealed exocyst requirements for several endocytic pathways including basolateral recycling, apical recycling, and basolateral-to-apical transcytosis. The latter was selectively dependent on interactions between the small GTPase Rab11a and Sec15A and was inhibited by expression of the C-terminus of Sec15A or down-regulation of Sec15A expression using shRNA. These results indicate that the exocyst complex may be a multipurpose regulator of endocytic traffic directed toward both poles of polarized epithelial cells and that transcytotic traffic is likely to require Rab11a-dependent recruitment and modulation of exocyst function, likely through interactions with Sec15A.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.